| 摘要: |
| 目的:按照过敏洗浴方的生产工艺制备过敏洗剂,测定过敏洗剂的高效液相色谱(HPLC)指纹图谱,为质量控制提供参考。 方法:色谱柱 Waters Sunfire C 18 (250 mm×4.6 mm,5 µm),乙腈为流动相 A,0.1%磷酸溶液为流动相 B,流速1.0 mL/min,检测波长 254 nm,柱温 30 ℃,进样量 10 µL,采用 HPLC 二元梯度洗脱法绘制过敏洗剂的指纹图谱。 结果:11 批过敏洗剂标定 15个共有峰,指认 5 个已知峰为绿原酸、升麻苷、木犀草苷、5-O-甲基维斯阿米醇苷和甘草酸。 结论:本研究建立的过敏洗剂 HPLC指纹图谱测定方法具有较好的专属性、重复性和实用性,色谱峰信息量大,为全面控制制剂的质量提供了可靠的方法。 |
| 关键词: 过敏洗剂 高效液相色谱 指纹图谱 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2018.05.015 |
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| 基金项目: |
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| Study on HPLC Fingerprint Chromatogram of Guomin Lotion |
| Xiao Miaosheng 1 , Zhu Haitao 1 , Zhang Youlin 1 , Yin Congcong 2 , Huang Liangyong 1 |
| (1. Shiyan Taihe Hospital Affiliated to Hubei University of Medicine, Hubei Shiyan 442000, China; 2. College of Pharmacy, Hubei University of Medicine, Hubei Shiyan 442000, China) |
| Abstract: |
| Objective: To measure the HPLC fingerprint chromatogram of Guomin lotion, provide reference for quality control.Methods: The separation was performed on a Waters Sunfire C 18 column (250 mm×4.6 mm, 5 µm), eluted with the mobile phase consisting of acetonitrile-0.1% phosphoric acid, the flow rate was 1.0 mL/min, the determine wavelength was 254 nm, the column temperature was 30 ℃. Results: The sample revealed that there were fifteen common peaks and five peaks were identificated to chlorogenic acid, prim-o-Gluccosylcimifugin, galuteolin, 5-O-methylvisammioside, glycyrrhizic acid. Conclusion: This method of HPLC fingerprint chromatogram have good specificity, repeatability and practicability. There is much information in chromatogram peaks, which provides credible method for controlling the quality Guomin lotion. |
| Key words: Guomin lotion HPLC fingerprints chromatogram |
