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| 孟鲁司特对不同阶段大鼠气管平滑肌细胞Ⅲ型胶原蛋白功能的影响 |
| 魏兵1,尚云晓2,孙铭蔚3,焦绪勇1,张超1 |
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| (1.沈阳军区总医院,辽宁沈阳 110016;2.中国医科大学附属盛京医院,辽宁沈
阳 110004;3.中国医科大学,辽宁沈阳 110122) |
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| 摘要: |
| [摘要]目的:探讨大鼠哮喘气道重塑过程中不同阶段气管平滑肌细胞(ASMC)分泌的Ⅲ型胶原蛋白功能的变化及孟鲁司特对Ⅲ型胶原蛋白的影响。方法:应用卵蛋白致敏激发建立哮喘气道重塑大鼠模型,原代培养ASMC,通过免疫组织化学方法检测ASMC中Ⅲ型胶原蛋白的表达。结果:哮喘组Ⅲ型胶原蛋白平均灰度值均高于对照组,以卵蛋白激发8周组最高,差异有统计学意义(P均<0.01);孟鲁司特干预组Ⅲ型胶原蛋白平均灰度值明显低于哮喘气道重塑组,尤以孟鲁司特干预2周及4周组差异更显著(P<0.01)。结论:孟鲁司特通过抑制ASMC分泌的Ⅲ型胶原蛋白合成能力而减轻气道重塑。 |
| 关键词: 哮喘 气道重塑 气管平滑肌细胞 Ⅲ型胶原蛋白 孟鲁司特 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2018.02.001 |
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| 基金项目:辽宁省科学技术计划面上项目,编号2015020444。 |
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| Effect of Montelukast on the Type Ⅲ Collagen in Rat Airway Smooth Muscle Cells at Different Stages |
| Wei Bing 1,Shang Yunxiao 2,Sun Mingwei 3,Jiao Xuyong 1,Zhang Chao 1 |
| (1.General Hospital of Shenyang Military Region,Liaoning Shenyang 110016, China; 2.Shengjing Hospital of China Medical University, Liaoning Shenyang 110004, China; 3.China Medical University, Liaoning Shenyang 110122, China) |
| Abstract: |
| [Abstract]Objective: To investigate the effect of montelukast on the secretion of type Ⅲ collagen in airway smooth muscle cells
(ASMC) during different stages of airway remodeling in asthmatic rats. Methods: Ovalbumin (OVA) was used to establish asthmatic
airway remodeling model of SD rats and montelukast was given to interfere. Primary culture purified ASMC, and measured the expression
of type Ⅲ collagen in ASMC by immunochemical method. Results: The average gray value of type Ⅲ collagen in asthma group was higher than that of the control group, and OVA motivated 8 weeks group was the highest. The differences were significant (P<0.01).The verage gray value of type Ⅲ collagen in montelukast group was significantly lower than that in asthmatic airway remodeling group,it was significantly different in 2 weeks group and 4 weeks group (P<0.01). Conclusion: Montelukast can reduce airway remodeling by inhibiting ASMC collagen synthesis ability. |
| Key words: asthma airway remodeling airway smooth muscle cell type Ⅲ collagen montelukast |
