| 摘要: |
| 目的:了解苏州地区儿童呼吸道人类博卡病毒(hBoV)混合感染的情况及混合感染后的临床特征。方法:选取2012-2015年苏州大学附属儿童医院呼吸科因呼吸道感染住院的患儿8 711例,收集鼻咽分泌物,应用实时PCR检测hBoV,逆转录-聚合酶链反应法(RT-PCR)检测人类偏肺病毒(hMPV)、人类鼻病毒(hRV),直接免疫荧光法检测呼吸道7种常见病毒包括呼吸道合胞病毒(RSV)、流感病毒A(InfA)、InfB、副流感病毒1 型(PinfⅠ)、PinfⅡ、PinfⅢ、腺病毒( ADV)。结果:8 711例标本中, hBoV阳性358例(4.11%)。358例hBoV阳性患儿中,92例(25.70%)存在混合感染,常见混合的病原为hRV、RSV和PinfⅢ。与单纯hBoV感染患儿相比,hBoV混合感染患儿月龄小(15.8个月 vs 20.3个月)、入院前病程长(12.1 d vs 9.5 d)、喘息发生率高(63.04% vs 48.12%)(P均<0.05),但两组患儿气促、呼吸困难、紫绀及住院时间比较差异均无统计学意义(P均>0.05)。结论:苏州地区儿童hBoV存在一定比例的混合感染,但混合感染并不加重病情。 |
| 关键词: 儿童 人类博卡病毒 混合感染 |
| DOI：doi: 10.13407/j.cnki.jpp.1672-108X.2017.12.003 |
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| 基金项目:国家自然基金项目,编号81573167;江苏省重点研发计划(社会发展)项目,编号BE2017657;苏州市科技项目,编号SYS201436 |
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| Clinical Research on Mixed Infection of Human Bocavirus in Children with Respiratory Tract |
| He Xiangping1, Wang Yuqing2 |
| (1. The Third People's Hospital of Kunshan, Jiangsu Suzhou 215300, China; 2. Children's Hospital of Soochow University, Jiangsu Suzhou 215003, China) |
| Abstract: |
| Objective: To investigate the situation and clinical features of human Bocavirus (hBoV) mixed infection of children in Suzhou area. Methods: From 2012 to 2015, a total of 8,711 children with respiratory tract infection were enrolled from department of respiration, Children's Hospital of Soochow University. We collected the nasopharyngeal secretions, and detected hBoV by real-time PCR. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect human Metapneumovirus (hMPV) and human Rhinovirus (hRV). Direct immunofluorescence method was used to detect seven common viruses in the respiratory tract including Respiratory syncytial virus (RSV), Influenza virus A (InfA), InfB, Parainfluenza virus type 1 (Pinf Ⅰ), Pinf Ⅱ, Pinf Ⅲ, Adenovirus (ADV). Results: Among the 8,711 samples, 358 cases were hBoV positive (4.11%), 92 cases (25.70%) were mixed infection, common mixed infection pathogens were hRV, RSV and Pinf Ⅲ. The mean month age of mixed infection was less than that of simple infection (15.8 months vs. 20.3 months). The duration of admission in children with mixed infection was longer than that in children with simple infection (12.1 d vs. 9.5 d). The incidence of asthma in children with mixed infection was higher than that in children with simple infection (63.04% vs. 48.12%, P<0.05), but there were no significant differences between shortness of breath, dyspnea, cyanosis and mean hospitalization day (P>0.05). Conclusion: There is a certain proportion of mixed infection in hBoV of Suzhou area,
but it does not aggravate the condition. |
| Key words: children human Bocavirus mixed infection |
