| 摘要: |
| 目的：建立小儿咳喘灵颗粒中6种主要活性成分的一测多评含量测定方法。方法：以绿原酸为内标物，采用高效液相色谱方法，建立其与其他5种成分新绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、4,5-O-二咖啡酰奎宁酸的相对校正因子，并用该校正因子进行计算，实现一测多评；同时采用外标法对6种成分进行含量测定，通过比较两种方法的结果，验证该方法的准确性。结果：建立的校正因子重复性较好，采用校正因子计算的含量值与外标法实测值偏差较好，相对误差<5%。结论：采用一测多评法测定小儿咳喘灵颗粒中6中绿原酸类成分可行。 |
| 关键词: 一测多评法 外标法 高效液相色谱法 校正因子 小儿咳喘灵颗粒 含量测定 |
| DOI：doi:10.13407/ j.cnki.jpp.1672-108X.2019.05.016 |
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| 基金项目:全国中药资源普查实施四川省试点项目,编号2014SS029-1。 |
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| Content Determination of Six Chlorogenic Acids in Xiao’er Kechuanling Granules by Quantitative Analysis of Multi-Components by Single-Marker |
| Zeng Zhen1, Liu Li1, Li Feng2, Wen Yongsheng1, Zhou Shiyu1, Xue Dan2 |
| (1. Chengdu Institute of Food and Drug Control, Sichuan Chengdu 610045, China; 2. West China School of Pharmacy Sichuan University, Sichuan Chengdu 610041, China) |
| Abstract: |
| Objective: To establish the quantitative analysis of multi-components by single-marker (QAMS) for content determination of six chlorogenic acids in Xiao’er Kechuanling granules. Methods: Emodin was used as the internal substance, HPLC method was adopted, the relative correction factors of emodin and neocyanogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, 4, 5-O-dicaffeyl quinine were established, and the correction factor was used to calculate the results, the QAMS was conducted. Meanwhile, the content of 6 components was determined by external standard method. The accuracy was verified by comparing the results of the two methods. Results: The established correction factor had good repeatability. The content calculated by the correction factor had better deviation from the measured value of the external standard method, with relative error <5%. Conclusion: It is feasible to determine the 6 chlorogenic acids in Xiao’er Kechuanling granules by QAMS. |
| Key words: quantitative analysis of multi-components by single-marker external standard method HPLC correction factor Xiao’er Kechuanling granules content determination |
