| 摘要: |
| 目的:通过对现有检测方法进行改良,将西门子VIVA-E生化分析仪对甲氨喋呤的检测灵敏度从0.30 µmol/L提升至0.05 µmol/L。方法:设置参数,降低试剂用量从180 µL 降至110 µL,同时更改定标点浓度为0、0.05、0.10、0.25、0.50 和1.00 µmol/ L,建立新方法。分别使用改良法、商业化方法及LC-MS/ MS法测量相同的样本进行对照检验。结果:改良后新方法在0.30-1.00 µmol/L范围内与商业化的方法有良好的相关性,在0.05-1.00 µmol/L范围内改良法与LC-MS/MS法有良好的相关性。结论:改良法在提高灵敏度的前提下将单个试剂盒的测量次数从140次提升至210次,显著降低了检测成本,且检测结果准确可靠,可在临床推广应用。 |
| 关键词: 甲氨蝶呤,血药浓度,酶放大免疫法,灵敏度 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2019.06.017 |
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| 基金项目: |
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| Improvement of Detection Sensitivity of Methotrexate by Modified Enzyme Multiplied Immunoassay Technique |
| Bing Haifeng, Li Zhong |
| (Dalian Children's Hospital, Liaoning Dalian 116012, China) |
| Abstract: |
| Objective: Through improving the existing detection method, to improve the sensitivity of the Siemens VIVA-E biochemical
analyzer to methotrexate from 0.30 µmol/ L to 0.05 µmol/ L. Methods: The new method was established by setting parameters,
reducing the reagent dosage from 180 µL to 110 µL, and changing the fixed punctuation concentration to 0, 0.05, 0.10, 0.25, 0.50
and 1.00 µmol/ L. The same samples were respectively measured by using the modified method, commercial method and LC-MS/MS
method for the control test. Results: The improved method had good correlation with the commercial method in the range of 0.30-1.00
µmol/L. The improved method had good correlation with LC-MS/MS in the range of 0.05-1.00 µmol/L. Conclusion: Under the premise
of improving sensitivity, the improved method increases the number of measurements of a single kit from 140 to 210, which greatly reduces the detection cost; and the detection results are accurate and reliable, and can be applied and promoted in clinical practice. |
| Key words: methotrexate blood drug concentration enzyme multiplied immunoassay technique sensitivity |
