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| 呼吸道合胞病毒与甲型流感病毒混合感染患儿血清炎症因子表达水平及临床意义 |
| 20220336 |
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| (Zhang Zhenzhen, Chen Junhua, Liu Chengjun, Li Yingcun, He Xiaoyan, Chen Dapeng, Zhan Xue, Zhang Zhiyong, Jia Yuntao, Li Yingliang, Liu Enmei, Hua Ziyu, Li Qiu, Xu Hongmei) |
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| 摘要: |
| 目的：探讨呼吸道合胞病毒（RSV）、季节性流感病毒A型（Inf-A）混合感染时患儿体内常见的炎症因子表达水平及其临床意义。方法：选取Inf-A和RSV检测阳性的患儿及部分健康儿童共115例纳入研究，将所有研究对象分为4组：Inf-A感染组、RSV感染组、混合感染组（MIX组）及健康对照组（HC组）。采用悬液芯片技术和酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）法分别检测4组患儿体内白介素IL-1β、IL-2、IL-8、肿瘤坏死因子TNF-α、干扰素IFN-γ等致炎性因子及IL-4、转化生长因子TGF-β等抗炎性细胞因子的表达水平，比较4组患儿的炎症因子水平与病程严重程度的相关性。结果：急性期Inf-A感染组、RSV感染组、MIX组患儿IL-1β、IL-8、TNF-α、IFN-γ水平均不同程度升高。RSV组IL-2较治疗前无变化，MIX组升高不明显。MIX组IL-1β、TNF-α、IL-8水平较治疗前均明显升高。在疾病恢复期，IL-1β、TNF-α、IL-8、IFN-γ水平较治疗前均明显下降，但与HC组相比TNF-α、IFN-γ水平仍明显偏高。RSV组、Inf-A组及MIX组IL-4与TGF-β均无明显升高，与HC组比较差异无统计学意义。结论：Inf-A、RSV混合感染患儿体内炎症因子占主导地位，以IL-1β、IL-8、TNF-α水平升高为主，而IL-1β、IL-8水平的变化可能是反应病情严重程度较敏感的指标。 |
| 关键词: 儿童 呼吸道病毒 混合感染 炎症因子 |
| DOI：doi:10.13407/ j.cnki.jpp.1672-108X.2019.10.001 |
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| 基金项目:2018 年度无锡市科教强卫工程医学重点学科,编号ZDXK12。 |
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| Expression and Clinical Significance of Serum Inflammatory Factors in Children with Respiratory Syncytial Virus and Influenza A Virus |
| 张祯祯,陈军华,刘成军,李英存,何晓燕,陈大鹏,詹学,张志勇,贾运涛,李映良,刘恩梅,华子瑜,李秋,许红梅 |
| (国家儿童健康与疾病临床医学研究中心,重庆医科大学附属儿童医院不明原因儿童严重急性肝炎诊治专家组,重庆 400014) |
| Abstract: |
| Objective: To probe into the expression levels and clinical significance of serum inflammatory factors in children with respiratory syncytial virus (RSV) and influenza A virus (Inf-A). Methods: A total of 95 children with positive Inf-A and RSV and some healthy children were enrolled in the study. All subjects were divided into 4 groups: Inf-A infection group, RSV infection group, mixed infection group (MIX group) and healthy control (HC group). Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of inflammatory factors such as IL-1β, IL-2, IL-8, TNF-α, IFN-γ and anti-inflammatory cytokines such as IL-4 and TGF-β in four groups. The correlation between inflammatory factor levels and severity of disease was compared and analyzed among four groups. Results: The levels of IL-1β, IL-8, TNF-α and IFN-γ in the Inf-A infection group, the RSV infection group and the MIX group increased to different degrees in the acute phase, and the levels of IL-1β, TNF-α and IL-8 in the MIX group increased more significantly. In the recovery period, the levels of IL-1β, TNF-α, IL-8 and IFN-γ were significantly lower than those before treatment, yet the levels of TNF-α and IFN-γ were still significantly higher than those in the HC group. Expression of IL-4 and TGF-β decreased significantly in the RSV group, the Inf-A group and the MIX group compared with before treatment, and there was no statistical significance compared with the HC group. Conclusion: The inflammatory factors increased predominantly in children infected with Inf-A and RSV, mainly concentrated in the levels of IL-1β, IL-8 and TNF-α, while the changes in the levels of IL-8 may be the sensitive indicators of the severity of the response. |
| Key words: children respiratory virus mixed infection inflammatory factors |
