| 摘要: |
| [摘要]目的：探讨泼尼松对脂多糖（LPS）诱导的支气管上皮细胞黏蛋白5AC（MUC5AC）、细胞间黏附分子-1（ICAM-1）、白细胞介素-6（IL-6）分泌的影响。方法：体外分离鉴定原代支气管上皮细胞，LPS以1 μg/mL剂量诱导细胞炎症反应，泼尼松5、10、20、40、50、100 μmol/L干预治疗，酶联免疫法（ELISA）检测细胞上清液MUC5AC、ICAM-1、IL-6水平变化，采用定量逆转录PCR（RT-PCR）、免疫印迹（Western-blot）检测MUC5AC、ICAM-1、IL-6 mRNA和蛋白表达情况，免疫荧光法检测核因子κB（NF-κB）核定位，Western-blot检测泼尼松干预治疗前后表皮生长因子受体（EGFR）、细胞外调节蛋白激酶1/2（ERK1/2）活化情况。结果：泼尼松以剂量依赖性降低LPS诱导的支气管上皮细胞内MUC5AC、ICAM-1、IL-6 mRNA水平和蛋白水平，抑制NF-κB、EGFR、ERK1/2活化。结论：泼尼松在体外能抑制MUC5AC、ICAM-1、IL-6的产生，其机制可能与抑制NF-κB、EGFR、ERK1/2的激活有关。 |
| 关键词: 支气管上皮细胞 泼尼松 黏蛋白5AC 细胞间黏附分子-1 白细胞介素-6 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2020.05.003 |
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| 基金项目: |
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| Effects of Prednisone on Secretion of Mucin-5 Subtype AC, Ntercellular Adhesion Molecule-1 and Interleukin-6 in Bronchial Epithelial Cells |
| Wang Xiaoqiu1, Zhang Pingbo1, Lu Ye1, Fang Feng1, Bao Yixiao1,2 |
| (1. Shanghai Pubin Children’s Hospital, Shanghai 200120, China; 2. National Children’s Medical Center, Shanghai Children’s Medical Center Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China) |
| Abstract: |
| [Abstract] Objective: To probe into the effects of prednisone on secretion of mucin-5 subtype AC (MUC5AC), intercellular adhesion molecule-1 (ICAM-1) and interleukin-6 (IL-6) in lipopolysaccharide (LPS) induced human bronchial epithelial cells. Methods: Primary human bronchial epithelial cells were isolated and identified in vitro. And 1 μg/mL of LPS was used to induce cell inflammatory response. And 5, 10, 20, 40, 50, 100 μmol/L of prednisone was used as intervention treatment. The levels of MUC5AC, ICAM-1 and IL-6 in cell supernatants was measured by euzymelinked immunosorbent assay (ELISA). The expression levels of MUC5AC, ICAM-1, IL-6 mRNA and protein were determined by real-time PCR (RT-PCR) and Western-blot. Immunofluorescence method was used to detect nuclear localization of nuclear factor-κB (NF-κB). Epidermal growth factor receptor (EGFR) and extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation before and after prednisone intervention were determined by Western-blot. Results: Prednisone inhibited the expression levels of MUC5AC, ICAM-1, IL-6 mRNA and protein in a dose-dependent manner in LPS induced human bronchial epithelial cells. Meanwhile, prednisone suppressed NF-κB, EGFR and ERK1/2 phosphorylation. Conclusion: In response to LPS induced inflammation, prednisone inhibits the expression levels of MUC5AC, ICAM-1 and IL-6 in vitro, which may be related to NF-κB, EGFR and ERK1/2 activation. |
| Key words: bronchial epithelial cells prednisone mucin-5 subtype AC intercellular adhesion molecule-1 interleukin-6 |
