| 摘要: |
| 目的：建立采高效液相色谱（HPLC）测定止痒消炎水中蛇床子素含量的方法。方法：采用HPLC法，以蛇床子素为目标成分，色谱柱为ZORBAX Eclipse Plus C18（250 mm×4.6 mm，5 μm），流动相为乙腈-水（65:35），流速为1.0 mL/min，柱温为35 ℃，检测波长为322 nm，进样量10 μL。结果：蛇床子素在15.936~63.744 μg/mL浓度范围内与峰面积积分值呈良好线性关系（r=0.999 9），平均回收率100.6%（n=6）。三批样品含量测定结果显示，本品每毫升含蛇床子素0.291 mg。结论：本方法专属性强，重现性好，结果准确，操作简便，可作为止痒消炎水中蛇床子素的含量测定方法 |
| 关键词: 止痒消炎水 蛇床子素 含量测定 高效液相色谱法 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2020.07.016 |
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| 基金项目: |
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| Determination of Osthole in Relieving Itching Anti-Inflammation Fluid by High Performance Liquid Chromatography |
| Zheng Weibing1, Zhao Dong1, Chen Zhipin2 |
| (1. Chongqing Xixuan Biotechnology Co., Ltd., Chongqing 408000, China; 2. Chongqing Shengsheng Pharmaceutical Co., Ltd., Chongqing 409100, China) |
| Abstract: |
| Objective: To establish the high performance liquid chromatography (HPLC) method for the determination of osthole in relieving itching anti-inflammation fluid. Methods: HPLC was used with osthole as the reference substance. The chromatographic column was ZORBAX Eclipse Plus C18 (250 mm×4.6 mm, 5 um). The mobile phase was acetonitrile-water (65∶35), the flow rate was 1.0 mL/min, the column temperature was 35 ℃, and the detection wavelength was 322 nm, with injection volume of 10 μL. Results: The linear range of osthol was 15.936 μg/mL to 63.744 μg/mL (r=0.999,9), and the average recovery was 100.6% (n=6). The average content of osthol in three batches of samples was 0.291 mg/mL. Conclusion: This method is highly specific, reproducible, reliable and simple, which can be used as the method for the determination of osthole in relieving itching anti-inflammation fluid |
| Key words: relieving itching anti-inflammation fluid osthole determination high performance liquid chromatography |
