| 摘要: |
| 目的：建立超高压液相色谱-串联质谱（UHPLC-MS/MS）法测定儿童血清中阿米卡星药物浓度的分析方法，并将该方法应用于临床样本的检测，指导临床合理用药。方法：血清样品经甲醇蛋白沉淀，以异帕米星为内标，采用Agilent Poroshell120 HILIC-Z（2.1 mm×100 mm，2.7 μm）色谱柱分离，流动相A：100 mM醋酸铵溶液（含1%甲酸），流动相B：乙腈（含1%甲酸），梯度洗脱。电喷雾离子源，多反应离子监测模式，采用正离子模式进行检测，检测离子通道为m/z 586.2/163.1（阿米卡星）和m/z 570.2/411.4（内标）。结果：阿米卡星在0.625~40.000 μg/mL范围内线性关系良好（r=0.999 6），日内和日间精密度<11.14%，准确度为91.76%~110.31%，基质效应95.02%~103.23%，提取回收率90.24%~96.29%。结论：本方法简单、快速、灵敏、准确，适用于人血清阿米卡星浓度分析测定。将该方法用于临床6例儿科患儿阿米卡星药物浓度监测，测定结果显示患儿的稳态谷浓度和峰浓度个体差异较大，提示阿米卡星在治疗过程中应进行药物浓度监测。 |
| 关键词: 阿米卡星 超高压液相色谱-串联质谱 治疗药物浓度监测 |
| DOI：doi:10.13407/j.cnki.jpp.1672.108X.2019.12.009 |
|
| 基金项目: |
|
| Ultra High Pressure Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Amikacin in Children Serum |
| Lin Zhiyan, Huang Xiaohui, Yang Ping, Lu Xiaotong |
| (Xinhua Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China) |
| Abstract: |
| Objective: To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for determination of amikacin in human plasma. The method was applied to clinical samples, especially for children. Methods: Samples were extracted with methanol, Isepamicin was used as internal standard (IS). The analysis was carried out on the Agilent Poroshell120 HILIC-Z (2.1×100 m, .7μm) column with gradient elution. The mobile phase was 100 mM ammonium acetate (containing 1% formic acid) and acetonitrile (containing 1% formic acid). The determination was performed on a triple quadrupole tandem mass spectrometer by multiple reactions monitoring (MRM) mode via electrospray ionization (ESI) source. The detection was performed in selected reaction monitoring mode at ion transition of m/z 586.2/163.1 for amikacin and m/z 570.2/411.4 for IS. Results: The standard curves were linear (r=0.999 6) over the concentration range of 0.625-40.000 μg/mL. The intra-day and inter-day precision was less than 11.14%; The accuracy ranged from 91.76% to 110.31%;The matrix effect ranged from 95.02% to 103.23%; The values of extraction recovery was from 90.23% to 96.29%. Conclusion: The determination method of amikacin was simple, rapid, sensitive and accurate, and was suitable for the TDM of amikacin. The method was applied to monitor the concentration of amikacin in 6 pediatric patients. The results showed that there were great individual differences in the concentration of amikacin. It was suggested that concentration of amikacin should be monitored during treatment. |
| Key words: amikacin UHPLC-MS/MS therapeutic drug monitoring |
