|
|
| |
|
|
| 本文已被：浏览 666次 下载 54次 |
码上扫一扫！ |
| 高效液相色谱法测定新生儿体内氟康唑的血药浓度 |
| 覃冬杰1,梁婧2,3,韦艳飞2,3,黄玉玲2,3,李萍2,3,江蓓2,3,诸葛明丽2,3,许峰2,3,王宏虹2,3,4,5,6,7 |
|
|
| (2,3,4,5,6,7(1. 柳州市质量检验
检测研究中心,广西柳州 545006;2. 柳州市妇幼保健院,广西柳州 545001;3. 广西科技大学附属妇产医院、儿童医院,广西柳
州 545001;4. 广西医科大学公共卫生学院,广西南宁 530021;5. 广西基因组与个体化医学重点实验室,广西南宁 530021;6. 广西基因组与个体化医学协同创新中心,广西南宁 530021;7. 广西高等学校重点实验室,广西南宁 530021)) |
|
| 摘要: |
| 目的：建立测定新生儿体内氟康唑血药浓度的高效液相色谱（HPLC）法。方法：HPLC色谱条件为色谱柱Diamonsil C18柱（5 μm，4.6mm×250 mm）；流动相甲醇﹕磷酸盐缓冲液（pH 7.0）=45︰55；检测波长261 nm；流速1 mL/min；柱温30℃；进样量10 μL。供试品溶液的制备，取样品加入内标后用乙酸乙酯萃取，有机相用氮气吹干，以甲醇溶解，进样测定。结果：该方法氟康唑在1.0~50.0 μg/mL范围内有良好线性关系，精密度、稳定性及回收率较好。结论：该方法操作简单、重复性好、准确可靠，可作为新生儿体内预防用氟康唑HPLC含量测定的方法。 |
| 关键词: 新生儿 氟康唑 含量测定 高效液相色谱 |
| DOI：doi:10.13407/j.cnki.jpp.1672.108X.2020.12.012 |
|
| 基金项目:广西自然科学基金青年基金项目，项目编号2018JJB140051；柳州市科技计划项目，项目编号2019BJ10603；柳州市科技计划项目，编号2018AF10501；广西卫健委自筹经费课题，编号Z2015186。 |
|
| Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography |
| Qin Donjie1, Liang Jing2,3, Wei Yanfei2,3, Huang Yuling2,3, Li Ping2,3, Jiang Bei2,3, Zhuge Mingli2,3, Xu Feng2,3, Wang Honghong2,3,4,5,6,7 |
| ((1. Liuzhou Quality Inspection and Testing Research Center, Guangxi Liuzhou 545006, China; 2. Liuzhou Maternity and Child Healthcare Hospital, Guangxi Liuzhou 545001, China; 3. Affiliated Maternity Hospital and Affiliated Children’s Hospital of Guangxi University of Science and Technology, Guangxi Liuzhou 545001, China; 4. School of Public Health Guangxi Medical University, Guangxi Nanning 530021, China;; 5. Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Nanning 530021, China; 6. Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Nanning 530021, China; 7. Guangxi Key Laboratory of Colleges and Universities, Guangxi Nanning 530021, China)) |
| Abstract: |
| Objective: To establish the high performance liquid chromatography (HPLC) method for determination of blood concentration of fluconazole in neonates. Methods: HPLC was carried out on Diamonsil C18 column (5 μm, 4.6 mm×250 mm). The mobile phase consisted of methanol and phosphate buffer solution (pH 7.0)=45︰55 at the flow of 1.0 mL/min, and the detection wavelength was 261 nm. Column temperature was 30 ℃ and sample amount was 10 μL. The blood sample with internal standard was extracted with ethyl acetate, the organic extract was concentrated to dryness under nitrogen, and the residue was dissolved in methanol and analyzed. Results: The standard curve was good linear from 1.0 to 50.0 μg/mL, and the precision, stability and recovery rate were good. Conclusion: This method is simple, repeatable, accurate and reliable, and can be used for determination of blood concentration of fluconazole in neonates by HPLC. |
| Key words: neonates fluconazole determination high performance liquid chromatography |
|
|
|
|
|
