| 摘要: |
| 目的：探讨乳酸杆菌对新生鼠和成年鼠辅助性T细胞（Th）1/Th2平衡及肺树突状细胞表型（DC）的影响。方法：新生鼠从出生后连续7 d每天经口给予109 CFU/mL浓度乳酸杆菌10 μL，成年鼠选取满6周的小鼠连续7 d每天经口给予109 CFU/mL浓度乳酸杆菌50 μL，实验第8天进行盲肠内容物培养以确定乳酸杆菌肠道定植情况。干预3周后，采用酶联免疫吸附试验（ELISA）检测血清干扰素（IFN）-γ、白细胞介素（IL）-4和IL-10，流式细胞仪检测肺树突状细胞亚型和成熟度。结果：（1）乳酸杆菌干预的新生鼠乳酸杆菌数量较对照组增加，而肠球菌和肠杆菌数量降低（P<0.01），乳酸杆菌干预的成年鼠肠道乳酸杆菌数量明显增加（P<0.01），但肠道肠球菌和肠杆菌数量没有变化。（2）乳酸杆菌干预的新生鼠IFN-γ和IL-10水平较对照组高（P<0.05），IL-4水平两组比较差异无统计学意义。乳酸杆菌干预的成年鼠IFN-γ、IL-4和IL-10水平与对照组比较差异无统计学意义。（3）乳酸杆菌干预的新生鼠肺CD11c+CD8α－水平为92.34%±0.94%，较对照组降低（P<0.05）；同时干预组CD11c+CD8α+水平较对照组增加（P<0.05）。经过乳酸杆菌干预后，新生鼠肺部14.03%±1.14%的DC表达MHCⅡ类分子，较对照组明显增加。成年鼠乳酸杆菌干预组CD11c+CD8α－、CD11c+CD8α+和CD11c+MHCⅡ+水平与对照组比较差异无统计学意义（P>0.05）。结论：乳酸杆菌干预可以促进新生鼠Th1/Th2平衡向Th1方向偏移，而对成年鼠没有影响。 |
| 关键词: 乳酸杆菌 辅助性T细胞1/辅助性T细胞2 树突状细胞 |
| DOI：doi:10.13407/ j.cnki.jpp.1672.108X.2021.09.001 |
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| 基金项目:四川省教育厅重点项目,编号16ZA0227;川北医学院重点项目,编号CBY13-A-ZD05。 |
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| Effects of Lactobacillus on Helper T Cell 1/Helper T Cell 2 Balance and Lung Dendritic Cell Phenotype of Neonatal Mice and Adult Mice |
| Xie Na1, Liu Chonghai2, Pi Guanghuan1, Lei Xunming1, Cai Yan1, Liu Haohao2, Li Xiaolin2 |
| (1. Sichuan Provincial Maternity and Child Health Hospital, Sichuan Chengdu 610031, China; 2. North Sichuan Medical College, Sichuan Nanchong 637000, China) |
| Abstract: |
| Objective: To probe into the effects of Lactobacillus on helper T cell 1 (Thl)/ helper T cell 2 (Th2) balance and lung dendritic cell (DC) phenotype of neonatal mice and adult mice. Methods: Neonatal mice were orally administered 10 μL Lactobacillus at the concentration of 109 CFU/mL every day for consecutive 7 d after birth. Adult mice at the age of 6 weeks were orally administered with 50 μL Lactobacillus at the concentration of 109 CFU/mL every day for consecutive 7 d. Culture of cecum contents was performed on the 8th day of the experiment to determine the intestinal colonization of Lactobacillus. After 3 weeks of intervention, serum interferon (IFN)-γ, interleukin (IL)-4 and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA), and lung dendritic cell subtype and maturation were detected by flow cytometry. Results: (1) Compared with the control group, the number of Lactobacillus in neonatal mice with Lactobacillus intervention increased, while the number of Enterococcus and Enterobacter decreased (P<0.01), the number of Lactobacillus in adult mice with Lactobacillus intervention were higher than those in the control group (P<0.01), yet the number of Enterococcus and Enterobacter in adult mice did not changed. (2) Compared with the control group, the levels of IFN-γ and IL-10 of neonatal mice with Lactobacillus intervention increased (P<0.05), there was no significant difference in the level of IL-4 between two groups. The difference was not statistically significant in the levels of IFN-γ, IL-4 and IL-10 between the adult mice with Lactobacillus intervention and the control group. (3) The level of CD11c+CD8α- of neonatal mice with Lactobacillus intervention was 92.34%±0.94%, lower than that of the control group (P<0.05), and the level of CD11c+CD8α+ in the intervention group was higher than that in the control group (P<0.05). After intervention by Lactobacillus, 14.03%±1.14% of DC in the lung of neonatal mice expressed MHCⅡ molecules, significantly higher than that in the control group. There was no significant difference in levels of CD11c+CD8α－, CD11c+CD8α+ and CD11c+MHCⅡ+ between the adult mice with Lactobacillus intervention group and the control group (P>0.05). Conclusion: Lactobacillus intervention can promote the shift in Th1/Th2 balance toward Th1 in neonatal mice, yet has no effect on adult mice. |
| Key words: Lactobacillus helper T cell 1/helper T cell 2 dendritic cell |
