| 摘要: |
| 目的：探讨五味子甲素对脂多糖诱导的小鼠RAW264.7细胞的抗炎作用。方法：检测五味子甲素对于脂多糖诱导的RAW264.7细胞的增殖、凋亡、吞噬及细胞内白细胞介素-lβ（IL-lβ）、肿瘤坏死因子-α（TNF-α）、前列腺素E2（PGE2）和环氧合酶-2（COX-2）水平的影响。脂多糖诱导后加入不同浓度五味子甲素，CCK8法测定RAW264.7细胞增殖，中性红试剂检测诱导后RAW264.7细胞的吞噬能力。经酶联免疫吸附试验（ELISA）法测定细胞培养血清中IL-lβ、TNF-α、PGE2和COX-2水平的表达。Annexin V-FITC/PI双染检测五味子甲素对诱导后RAW264.7细胞凋亡的影响。结果：五味子甲素可明显抑制脂多糖诱导的RAW264.7细胞的增殖、吞噬能力及诱导刺激的细胞凋亡；ELISA结果显示，五味子甲素可以抑制细胞IL-lβ、TNF-α、PGE2和COX-2的水平。结论：五味子甲素对脂多糖诱导的小鼠RAW264.7细胞炎症具有明显的抑制作用，其机制可能与抑制炎症细胞的增殖、吞噬，减少炎症因子释放，减轻脂多糖诱导引起的细胞凋亡有关。 |
| 关键词: 五味子甲素 RAW264.7细胞增殖 吞噬能力 抗炎作用 |
| DOI：doi:10.13407/ j.cnki.jpp.1672.108X.2022.08.001 |
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| 基金项目:辽宁省自然科学基金,编号2019-ZD-0441 |
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| Anti-Inflammatory Effects of Schisandrin A on Lipopolysaccharide-Induced RAW264.7 Cells in Mice |
| Xu Hong, Tao Xiaojun, Li Ruonan, Li Xinlei, Xu Zhili |
| (College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Liaoning Dalian 116600, China) |
| Abstract: |
| Objective: To probe into the anti-inflammatory effects of schisandrin A on lipopolysaccharide-induced RAW264.7 cells in mice. Methods: Effects of schisandrin A on proliferation, apoptosis and phagocytosis of lipopolysaccharide-induced RAW264.7 cells in mice and levels of interleukin (IL)-1β, tumor necrosis factor-α (TNF)-α, prostaglandin E2 (PGE2) and cyclooxygenase (COX)-2 levels were detected. The proliferation of RAW264.7 cells was measured by CCK8 method and the phagocytosis of RAW264.7 cells was detected by neutral red reagent. IL-1β, TNF-α, PGE2 and COX-2 were determined by enzyme-linked immunosorbent assay (ELISA). Annexin V-FITC/PI double staining was used to detect the effects of schisandrin A on apoptosis of RAW264.7 cells after induction. Results: Schisandrin A could significantly inhibit the proliferation, phagocytosis and apoptosis of lipopolysaccharide-induced RAW264.7 cells in mice. ELISA results showed that schisandrin A could inhibit the secretion of IL-lβ, TNF-α, PGE2 and COX-2 in RAW264.7 cells. Conclusion: Schisandrin A has significant anti-inflammatory effects on lipopolysaccharide-induced RAW264.7 cells in mice, and its mechanism may be related to inhibiting the proliferation and phagocytosis of inflammatory cells, reducing the release of inflammatory factors, and reliefing the lipopolysaccharide-induced apoptosis. |
| Key words: schisandrin A RAW264.7 cells proliferation phagocytosis ability anti-inflammatory effects |
