| 摘要: |
| 目的:探讨益生菌对食源性过敏(FA)婴儿肠道微生态的影响。 方法:选取 2020-2022 年我院 67 例 6 月龄以下 FA 婴儿, 按监护人自愿原则设为益生菌组 37 例及未干预组 30 例,并选取同期 38 例健康婴儿为对照组。 采用高通量测序检测研究对象 的粪便,了解肠道菌群多样性及差异性。 结果:未干预组 Shannon 指数、细菌群落多样性参考指标临时操作分类单元(OTU)高 于益生菌组及对照组,Simpson 指数低于益生菌组及对照组,差异有统计学意义(P<0. 05)。 未干预组厚壁菌门丰度、变形菌门 丰度均高于对照组及益生菌组(P<0. 05)。 未干预组双歧杆菌属丰度降低,链球菌属丰度、未分类肠杆菌属丰度、肠球菌属丰 度、柠檬酸杆菌属、狭义梭菌-1 属丰度均高于对照组及益生菌组(P<0. 05)。 结论:FA 与肠道微生态关系密切,益生菌对 FA 婴 儿肠道微生态有一定改善作用。 |
| 关键词: 益生菌 食源性过敏 婴儿 喂养方式 高通量测序 肠道微生态 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2024.07.011 |
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| 基金项目:杭州市科技局项目,编号 B20231535。 |
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| Clinical Research on Effect of Probiotics on Intestinal Microecology of Food-Allergic Infants |
| Ying Wenyue, Jia Shuwen |
| ((Linping Maternal and Child Health Hospital, Hangzhou 311113, China)) |
| Abstract: |
| Objective: To probe into the effects of probiotics on intestinal microecology of infants with food allergy (FA). Methods: A total of 67 infants with FA under 6 months old in our hospital were extracted as probiotic group (37 cases) and non-intervention group (30 cases) according to the principle of voluntariness, and 38 healthy infants in the same period were extracted as the control group. High-throughput sequencing was used to detect the stool of the study subjects to investigate the diversity and difference of intestinal flora. Results: The Shannon index and operational taxonomic unit (OTU) in the non-intervention group were higher than those in the probiotic group and the control group, and the Simpson index was lower than those in the probiotic group and control group, with statistically significant differences (P<0. 05). The abundances of firmicutes and proteobacteria in non-intervention group were higher than those in control group and probiotic group ( P < 0. 05). The abundance of Bifidobacterium decreased in the non-intervention group, and the abundance of Streptococcus, unclassified Enterobacterium, Enterococcus, Citrobacter and Clostridium-1 were higher than those in the control group and probiotic group (P<0. 05). Conclusion: FA is closely related to intestinal microecology, and probiotics can improve the intestinal microecology of FA infants to some extent. |
| Key words: probiotics food allergy infants feeding mode high-throughput sequencing intestinal microecology |
