| 摘要: |
| 目的:基于数据挖掘及网络药理学方法,探讨吴力群教授治疗儿童过敏性鼻炎哮喘综合征(CARAS)用药规律,分析核心
组方的分子作用机制。方法:将收集的CARAS 病例录入临床科研信息共享系统,采用复杂网络分析获取吴力群治疗儿童
CARAS 核心组方。应用中药系统药理学数据库与分析平台( TCMSP) 获取核心组方药物靶点,使用MalaCards、GeneCards、
OMIM、DrugBank 数据库分别获取过敏性鼻炎和哮喘疾病靶点取并集。应用CytoScape 3. 7. 1 及STRING 11. 0 平台构建中药活
性成分-靶点、蛋白相互作用( PPI) 网络,借助MetaScape 对共同靶点进行基因本体论( GO) 及京都基因与基因组百科全书
(KEGG)富集分析,并通过微生信绘制柱形图和气泡图。结果:共收集140 例CARAS 患儿878 诊次处方,涉及中药177 种,药性
以温、平、寒居多,药味以甘、苦、辛为主,归经以肺经、脾经、胃经为主,通过复杂网络共获取11 味核心组方。其中核心组方药物
活性成分46 种,涉及254 个靶点,疾病靶点984 个,交集靶点112 个。结论:吴力群治疗儿童CARAS 核心组方为法半夏、陈皮、
茯苓、瓜蒌、炒莱菔子、白芷、辛夷、防风、银柴胡、乌梅、醋五味子等,主要通过多成分、多靶点、多途径发挥治疗CARAS 作用,为
后续实验研究及临床应用提供参考。 |
| 关键词: 过敏性鼻炎哮喘综合征 数据挖掘 网络药理学 儿童 |
| DOI：doi:10.13407/j.cnki. jpp.1672-108X.2024.11.003 |
|
| 基金项目:基金项目:北京市中医药科技发展资金项目,编号JJ-2023-49;北京中医药大学新教师启动基金项目,编号2020-JYB-XJSJJ-053;北京中医药
大学新教师启动基金项目,编号2021-JYB-XJSJJ-073。 |
|
| Core Prescriptions of Wu Liqun in the Treatment of Combined Allergic Rhinitis and Asthma Syndrome inChildren and Its Molecular Mechanism |
| Li Panpan1,Gao Shiyu2, Zhang Ye3, Huo Jingwei4,Chen Haipeng4, Wu Liqun4 |
| (1. The First Affiliated Hospital of
Tsinghua University, Beijing 100015, China; 2. Shenzhen Hospital, Beijing University of Chinese Medicine, Guangdong Shenzhen
518172, China; 3. Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming 650032, China; 4. Oriental Hospital,
Beijing University of Chinese Medicine, Beijing 100071, China) |
| Abstract: |
| Objective: To explore the medication rules of Professor Wu Liqun in the treatment of combined allergic rhinitis and asthma
syndrome (CARAS) in children and analyze the molecular mechanism of core prescriptions based on data mining and network
pharmacology. Methods: The collected CARAS cases were entered into the clinical research information sharing system, and the core
prescriptions were obtained by complex network analysis. Traditional Chinese Medicines Systems Pharmacology Platform (TCMSP) was
used to obtain the core drug targets, MalaCards, GeneCards, OMIM and DrugBank were used to obtain the disease targets of allergic
rhinitis and asthma, respectively. CytoScape 3. 7. 1 and STRING 11. 0 platform were used to construct the active component-target,
protein-protein interaction (PPI) network of traditional Chinese medicine. The common targets were analyzed by Gene Ontology (GO)
and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis based on MetaScape. The microbioinformatics was used to
draw bar charts and bubble charts. Results: A total of 878 prescriptions were collected from 140 children with CARAS, including 177 kinds of
traditional Chinese medicine. The properties of the medicine were mostly warm, mild and cold. The taste of the medicine was mainly
sweet, bitter and pungent, and the meridians were mainly lung, spleen and stomach. A total of 11 core prescriptions were obtained
through complex network. There were 46 active components of core prescriptions, including 254 targets, 984 disease targets and
112 intersection targets. Conclusion: Core prescriptions of Wu Liqun in the treatment of CARAS are Rhizoma pinelliae
preparatumpinelliae, Citrus reticulata, Poria cocos Wolf, Trichosanthes kirilowii maxim, roasted Raphani semen, Angelicae dahuricae
radix, Magnoliae flos, Saposhnikoviae radix, Gypsophila licentiana Hand. -Mazz. , Mume fructus, and Schisandra vinegar, which mainly
play a role in the treatment of CARAS through multiple components, multiple targets and multiple ways, providing reference for
subsequent experimental research and clinical application. |
| Key words: combined allergic rhinitis and asthma syndrome data mining network pharmacology children |
