| 摘要: |
| 目的:探讨溃疡性结肠炎(UC)患儿血清肿瘤坏死因子受体相关因子3(TRAF3)、半乳糖凝集素-3(Gal-3)、过氧化物氧化
还原酶蛋白1(PRDX1)水平与病情、预后的关系。方法:选取我院2022 年4 月至2023 年4 月收治的UC 患儿(UC 组)和体检健
康儿童(对照组)各176 例。根据梅奥内镜评分(MES)将UC 组分为MES Ⅰ级63 例,MES Ⅱ级71 例和MES Ⅲ级42 例;根据
2 年随访结局分为预后良好组121 例和预后不良组55 例。采用酶联免疫吸附试验检测血清TRAF3、Gal-3、PRDX1 水平;采用
Pearson 或Spearman 相关性分析、多因素logistic 回归、ROC 曲线分析,Bootstrap 法进行内部验证。结果:与对照组相比,UC 组血
清TRAF3、PRDX1 水平下降,Gal-3 水平升高(P<0. 05)。MES Ⅰ~Ⅲ级患儿血清TRAF3、PRDX1 水平依次降低,Gal-3 水平依次
升高(P<0. 05)。预后不良组血清TNF-α、Gal-3 水平高于预后良好组,SOD、TRAF3、PRDX1 水平低于预后良好组(P<0. 05)。血
清TRAF3、PRDX1 与Gal-3、MES 分级、TNF-α 呈负相关,与SOD 呈正相关;Gal-3 与MES 分级、TNF-α 呈正相关,与SOD 呈负相
关;血清TRAF3 与PRDX1 呈正相关(P<0. 05)。TNF-α、Gal-3、SOD、TRAF3、PRDX1 为预后不良的影响因素(P<0. 05)。三者联
合预测预后不良的AUC 优于单一预测。Bootstrap 校正后AUC 为0. 927(95%CI 0. 883 ~ 0. 958),Hosmer-Lemeshow 检验χ2 =
6. 324,P =0. 612。结论:UC 患儿血清TRAF3、PRDX1 水平降低,Gal-3 水平升高,三者与疾病活动度分级密切相关,联合预测预
后不良的价值较高。 |
| 关键词: 溃疡性结肠炎 肿瘤坏死因子受体相关因子3 半乳糖凝集素-3 过氧化物氧化还原酶蛋白1 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2026.10.011 |
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| 基金项目: |
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| Development of Prognostic Prediction Model for Pediatric Ulcerative Colitis Based on Levels of TumorNecrosis Factor Receptor Associated Factor 3, Galectin-3, and Peroxidative Oxidoreductase Protein 1 |
| Xue Baizhao, Li Xiaona, Zhang Jinjin, Wang Hongling, An Hongxian, Zheng Rongjuan |
| ((Tangshan Central Hospital, Hebei Tangshan 063000, China)) |
| Abstract: |
| Objective: To investigate the correlation between levels of tumor necrosis factor receptor-associated factor 3 (TRAF3),
galectin-3 (Gal-3), and peroxiredoxin 1 (PRDX1) and disease severity, prognosis in children with ulcerative colitis (UC). Methods:
From Apr. 2022 to Apr. 2023, totally 176 children with UC (UC group) and 176 children undergoing routine health checkups (control
group) in our hospital were enrolled. Based on the Mayo Endoscopic Score (MES), the UC group was classified as follows: 63 cases
with MES grade Ⅰ, 71 cases with MES grade Ⅱ, and 42 cases MES grade Ⅲ. Based on 2-year follow-up outcomes, UC group was
further divided into the good prognosis group (121 cases) and poor prognosis group (55 cases). Enzyme-linked immunosorbent assay
was used to detect serum levels of TRAF3, Gal-3, and PRDX1. Pearson or Spearman correlation analysis, multivariate logistic
regression, ROC curve analysis, and the Bootstrap method were employed for internal validation. Results: Compared with the control
group, the UC group showed decreased serum TRAF3 and PRDX1 levels and elevated Gal-3 levels (P<0. 05). The levels of serum
TRAF3 and PRDX1 in children with MES grade Ⅰ to Ⅲ decreased successively, while the level of Gal-3 increased successively (P<0. 05).
The levels of TNF-α and Gal-3 in the poor prognosis group were higher than those in good prognosis group, while the levels of SOD,
TRAF3 and PRDX1 were lower in poor prognosis group (P<0. 05). Serum TRAF3, PRDX1 were negatively correlated with Gal-3, MES
grade, and TNF-α, and positively correlated with SOD. Gal-3 was positively correlated with MES grade and TNF-α, and negatively
correlated with SOD. Serum TRAF3 was positively correlated with PRDX1 (P<0. 05). TNF-α, Gal-3, SOD, TRAF3, and PRDX1 were
identified as risk factors for poor prognosis (P<0. 05). The AUC for predicting poor prognosis using the three variables in combination
was superior to that of any single predictor. The AUC after bootstrap correction was 0. 927 (95% CI 0. 883 to 0. 958); the Hosmer-
Lemeshow test yielded χ2 =6. 324, P = 0. 612. Conclusion: Children with UC have reduced serum levels of TRAF3 and PRDX1 and
elevated levels of Gal-3, all three markers are closely associated with disease activity scores and are highly valuable for predicting poor
prognosis when used in combination. |
| Key words: ulcerative colitis tumor necrosis factor receptor associated factor 3 galectin-3 peroxidative oxidoreductase protein 1 |
