| 摘要: |
| 目的:建立槟榔花口服液中槟榔碱的高效液相色谱测定方法。方法:以安捷伦ZORBAX SB-C18 色谱柱(250 mm×4. 6 mm,
5 μm)为色谱柱,以乙腈-0. 01%磷酸溶液(每1 000 mL 含2. 5 g 十二烷基硫酸钠)(32 ∶ 68)为流动相,波长215 nm,柱温30 ℃,流速
1. 0 mL/ min,进样量5 μL。结果:氢溴酸槟榔碱进样量在0. 025 2~0. 504 5 μg 范围内,与峰面积呈良好的线性关系;平均加样回收
率为101. 62%,相对标准偏差(RSD)= 1. 20%(n =9)。结论:该方法简单、准确,重复性好,可用于槟榔花口服液的质量控制。 |
| 关键词: 槟榔碱 氢溴酸槟榔碱 槟榔花 槟榔花口服液 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2026.10.006 |
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| 基金项目: |
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| Determination of Arecoline in Areca Inflorescence Oral Liquid by High Performance Liquid Chromatography |
| Zheng Weibing, Chen Zhiping, Luo Haixia, Yang Li |
| ((Hainan Jushen Pharmaceutical Co. , Ltd. , Haikou 571157, China)) |
| Abstract: |
| Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of arecoline in areca
inflorescence oral liquid. Methods: The separation was performed on an Agilent ZORBAX SB-C18 column (250 mm × 4. 6 mm, 5 μm).
The mobile phase consisted of acetonitrile-0. 01% phosphoric acid solution (2. 5 g sodium dodecyl sulfate per 1 000 mL) at a volume
ratio of 32 ∶ 68. The detection wavelength was set at 215 nm, the column temperature was 30 ℃, the flow rate was 1. 0 mL/ min, and
the injection volume was 5 μL. Results: Arecoline hydrobromide showed a good linear relationship with the peak area in the range of
0. 025 2 to 0. 504 5 μg. The average sample recovery rate was 101. 62% with relative standard deviation (RSD)= 1. 20% (n = 9).
Conclusion: This method is simple, accurate and reproducible, which can be used for the quality control of areca inflorescence oral liquid. |
| Key words: arecoline arecoline hydrobromide areca inflorescence areca inflorescence oral liquid |
