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| pNaKtide 对卵清蛋白诱导哮喘大鼠气道炎症及细胞因子与Th1/ Th2
免疫失衡的影响 |
| 万霞1,鲁娜1,谢慧如1,胡泽功1,冯星星1,金润铭1,刘光辉2,白燕1,2 |
| (1. 华中科技大学同济医学院附属协和医院,
武汉 430022; 2. 香港中文大学(深圳)医院,广东深圳 518129) |
|
| 摘要: |
| 目的:评价pNaKtide 对卵清蛋白(OVA)诱导哮喘大鼠气道炎症、氧化应激及Th1/ Th2 免疫失衡的影响。方法:将20 只
SD 大鼠随机分为对照组、哮喘组、布地奈德组和pNaKtide 组,每组各5 只。采用OVA 致敏和雾化激发建立哮喘模型,观察肺组
织病理改变,检测支气管肺泡灌洗液(BALF)中嗜酸性粒细胞百分比和细胞因子水平、肺组织氧化应激指标及GATA 结合蛋白
3(GATA-3)和T 盒转录因子( T-bet) 蛋白表达,并以流式细胞术检测外周血Th1、Th2 细胞百分比。结果:与哮喘组相比,
pNaKtide 组BALF 中嗜酸性粒细胞百分比及IL-3、IL-4、IL-5、IL-17 和TNF-α 水平降低,IL-10 水平升高;肺组织谷胱甘肽(GSH)、
超氧化物歧化酶( SOD) 和总抗氧化能力( T-AOC) 水平升高,丙二醛( MDA) 水平降低;GATA-3 表达降低、T-bet 表达升高,
Th1/ Th2 免疫失衡有所改善。结论:pNaKtide 可减轻OVA 诱导哮喘大鼠的气道炎症和氧化应激,并改善Th1/ Th2 免疫失衡,其
具体作用机制仍需进一步研究。 |
| 关键词: 哮喘 pNaKtide 氧化应激 Th1/ Th2 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2026.11.003 |
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| 基金项目:基金项目:国家自然科学基金,编号82070023;湖北儿科联盟医学研究项目,编号HPAMRP202105。 |
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| Effects of pNaKtide on Airway Inflammation and Cytokine Levels and Th1 / Th2 Immune Imbalance inAsthmatic Rats Induced by Ovalbumin |
| Wan Xia1, Lu Na1, Xie Huiru1, Hu Zegong1, Feng Xingxing1, Jin Runming1, Liu Guanghui2, Bai Yan1 |
| (1. Union
Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; 2. Medical Centre of the
Chinese University of Hong Kong, Shenzhen, Guangdong Shenzhen 518129, China) |
| Abstract: |
| Objective: To evaluate the effects of pNaKtide on airway inflammation, oxidative stress, and Th1/ Th2 imbalance in rats
with ovalbumin (OVA)-induced asthma. Methods: Twenty male Sprague-Dawley rats were randomly assigned to the control group,
asthma group, budesonide group, and pNaKtide group, with 5 cases in each group. Asthma was induced by OVA sensitisation and
aerosol challenge. Lung histopathology, eosinophil percentages and cytokine levels in bronchoalveolar lavage fluid, oxidative-stress
indices and GATA-binding protein 3 (GATA-3) and transcription factor T-bet protein expression in lung tissue were observed. And the
proportion of Th1 and Th2 cells in peripheral blood was detected by flow cytometry. Results: Compared with the asthma group, the
pNaKtide group showed lower eosinophil percentages, lower IL-3, IL-4, IL-5, IL-17, and TNF-α levels, and higher IL-10 level.
Glutathione (GSH), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) levels increased, whereas malonaldehyde
(MDA) levels decreased in pNaKtide group. GATA-3 expression decreased, T-bet expression increased, and the Th1/ Th2 imbalance
was partially corrected. Conclusion: pNaKtide can alleviate airway inflammation and oxidative stress in rats with OVA-induced asthma,
and improve the Th1/ Th2 immune imbalance. The specific mechanism still requires further investigation. |
| Key words: Keywords]asthma pNaKtide oxidative stress Th1/ Th2 |
