| 摘要: |
| 目的:建立荔大前合剂的质量标准。方法:取荔大前合剂样品,采用薄层色谱(TLC)法对车前草做定性鉴别;采用高效液相色谱(HPLC)法[色谱柱:Agilent-C18(150 mm×4.6 mm,5 μm);流动相:甲醇-0.5% 醋酸(55∶45);检测波长:326 nm;进样量:20 μL]测定蒙花苷含量。结果:荔大前合剂与车前草对照药材在TLC平板相应位置显现相同颜色的斑点,且阴性对照无干扰。蒙花苷在1.217~7.303 μg/mL浓度范围内与峰面积呈良好线性关系(r=0.999 6),加样回收率99.54%(RSD=1.5%,n=9)。结论:该方法简便易行,准确性和专属性好,可用于荔大前合剂的质量控制。 |
| 关键词: 荔大前合剂 质量标准 车前草 薄层色谱 蒙花苷 高效液相色谱法 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2017.08.017 |
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| 基金项目: |
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| Quality Standard of Lidaqian Mixture |
| Jing Xia, Xu Jing, Xu Zejun, Liu Yao |
| (Children's Hospital of Nanjing Medical University, Jiangsu Nanjing 210008, China) |
| Abstract: |
| Objective: To establish the quality standard of Lidaqian mixture. Methods: Plantago depressa Willd. was identified by thin layer chromatography (TLC). HPLC was adopted to determine the content of buddleoglucoside in Lidaqian mixture. The Agilent-C18 column (150 mm×4.6 mm, 5 μm) was used with the mobile phase of methanol-0.5% acetic acid (55∶45), the detection wavelength was at 326 nm. Results: TLC identification method was simple and feasible with specificity. The calibration curve was linear over the ranges of 1.217 to 7.303 μg/mL (r=0.999,6) for buddleoglucoside when the sample volumn was 20 μL. The average recovery was 99.54% , RSD was 1.5% (n=9). Conclusion: The method was simple, accurate and specific for quality control of Lidaqian mixture. |
| Key words: Lidaqian mixture quality standard Plantago depressa Willd. TLC buddleoglucoside HPLC method |
