| 摘要: |
| 目的:建立测定补肾益脑片中梓醇含量的方法。方法:采用高效液相色谱法测定,Amethyst C18-H 色谱柱(4.6 mm×150 mm,5 μm),柱温为室温,流动相为乙腈-0.1%磷酸水溶液(3∶97),流速为0.8 mL/min,检测波长为205 nm。结果:补肾益脑片中的梓醇在2~100 μg/mL 线性范围内与峰面积线性关系良好,相关系数为0.999 6,平均回收率为95.8%(n= 5),RSD为2.15%。结论:本方法简便、快速,可用于补肾益脑片中梓醇的含量测定。 |
| 关键词: 补肾益脑片 梓醇 含量测定 高效液相色谱法 |
| DOI：doi: 10.13407/j.cnki.jpp.1672-108X.2017.11.016 |
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| 基金项目:湖北医药学院校基金立项资助项目,编号FDFR201614;湖北省卫计委指导性项目,编号WJ2017F082;湖北省教育厅科学技术研究项目(中青年人才项目),编号Q20162113 |
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| Determination of Catalpol in Bushen Yinao Tablet by HPLC Method |
| Luo Xingxun 1, Wang Hongmei 2, Chen Qinhua 2 |
| (1. Affiliated Suizhou Hospital, Hubei University of Medicine, Hubei Suizhou 441300, China; 2. Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei Shiyan 442008, China) |
| Abstract: |
| Objective: To develop a HPLC method for determination the content of catalpol in Bushen Yinao tablet. Methods: The HPLC method was established, Amethyst C18-H column (4.6 mm×150 mm, 5 μm) was chosen as a chromatographic column, with the column temperature of room temperature and acetonitrile-0.1% glacial acetic acid in water (3∶97) as mobile phase. The flow rate was 0.8 mL/min and detection wavelength was at 205 nm. Results: The good linearity range of 2~100 μg/mL and r=0.999,6 were obtained. And the main recovery was 95.8% (n=5), with RSD was 2.15%. Conclusion: This method is simple, rapid and it can be used for determination of catalpol in Bushen Yinao tablet. |
| Key words: Bushen Yinao tablet catalpol content determination HPLC |
