| 摘要: |
| 目的：探讨三门地区儿童呼吸道流感嗜血杆菌（Hi）的生物分型及耐药性。方法：回顾性分析2013年1月至2014年12月在三门县人民医院儿科门诊与住院部治疗的呼吸道感染患儿7 140例，采用无菌负压吸引法采集新鲜痰液，进行流感嗜血杆菌培养、分离，进行生物分型，使用生物鉴定仪、鉴定卡、药敏试剂盒进行药敏试验并对其β-内酰胺酶进行检测。结果：7 140例儿童呼吸道感染患儿分离出337株Hi；生物学分型以Ⅱ型和Ⅲ型所占比例最大，二者占全部Hi菌株的91.0%；337株Hi β-内酰胺酶阳性率为32.64%（110/337）；Hi的各类抗菌药物耐药性检测中氨苄西林的耐药率最高；Hi对阿莫西林/克拉维酸、四环素、头孢噻肟、利福平、氯霉素等的敏感率均大于90%，对氧氟沙星、头孢呋辛、头孢克洛等敏感率均大于80%。结论：2013年至2014年三门地区儿童呼吸道流Hi生物学分型以Ⅱ型、Ⅲ型为主，且Ⅱ型产β-内酰胺酶的几率最高，Hi对氨苄西林的耐药率最高，对阿莫西林/克拉维酸、四环素、头孢噻肟、利福平、氯霉素等的敏感率均大于90%，阿莫西林/克拉维酸是治疗Hi感染的首选药物。 |
| 关键词: 儿童 流感嗜血杆菌 耐药性 生物学分型 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2016.04.015 |
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| 基金项目: |
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| Analysis of the Biological and Drug Resistance of the Respiratory Tract Haemophilus Influenzae in Children in Sanmen Area from 2012 to 2014 |
| Ding Zhengxiang, Wang Changyi, Xu Xiaohong |
| (Sanmen Ppeople's Hospital of Zhejiang Province, Zhejiang Sanmen 317100, China) |
| Abstract: |
| Objective: To analyze the status and drug resistance of the biological typing of respiratory tract Haemophilus influenzae (HI) of children in Sanmen area. Methods: Retrospective analysis between January 2013 and December 2013, our hospital pediatric outpatient and the inpatient treatment of 7, 140 cases of children with respiratory tract infection, adopting aseptic pressure suction method to collect fresh sputum, h. influenzae culture, separation, biological classification, using the biological identification, identification card, susceptibility kits for drug sensitive test and the beta lactamase for testing. Results: 7140 cases of children with respiratory tract infection in children with isolated 337 strains of Hi, the isolation rate was 4.72%; biological type was type II and type III accounted for the largest proportion, the two accounted for all isolates were 91.0%; 337 strains Hi total beta lactamase positive rate was 32.64% (110/337); Hi all kinds of antibacterial drug resistance testing in, benzyl chloride methicillin resistant rate was the highest, the sensitivity rate of amoxicillin / g column dimensional breaking, tetracycline, cefotaxime cefotaxime, rifampicin, chloramphenicol and other hi were greater than 90%, the rate of oxygen floxacin Buddha, cefuroxime, cefaclor and sensitivity to Hi were greater than 80%. Conclusion: From 2013 to 2014 Sanmen area children with respiratory tract Hi biological type dominated by type II and III, and type II producing beta lactamase, the highest rate hi of chloro benzyl penicillin resistance rate was the highest, the sensitivity rate of amoxicillin/clavulanic acid, tetracycline, cefotaxime cefotaxime, rifampicin, chloramphenicol were greater than 90%, the sensitivity rate of amoxicillin/clavulanic acid is the drug of choice for treatment of Hi infection. |
| Key words: children Haemophilus influenzae drug resistance biological typing |
