| 摘要: |
| 目的:建立淡竹叶颗粒中荭草苷和异荭草苷的含量测定方法。方法:采用FORTIS-C18柱(4.6 mm×250 mm,5 μm),以乙腈-0.2%磷酸溶液为流动相,采用梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长330 nm。结果:荭草苷和异荭草苷分别在3.08 ~61.50 μg/mL和2.18~43.60 μg/mL浓度范围内与峰面积有良好线性关系(r=0.999 9),平均加样回收率分别为99.1%、98.6%,RSD分别为0.94%、0.90%。结论:本方法专属性强,灵敏度高,重复性好,可作为控制淡竹叶颗粒质量的方法。 |
| 关键词: 荭草苷 异荭草苷 淡竹叶颗粒 高效液相色谱法 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2016.07.016 |
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| 基金项目: |
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| Determination of Orientin and Isorientin in Danzhuye Granule |
| Feng Wanqi, Li Zhihao, Qu Jinghong |
| (Affiliated Dongfeng Hospital of Hubei University of Medicine, Hubei Shiyan 442008, China) |
| Abstract: |
| Objective: To establish a method for the determination of orientin and isorientin in Danzhuye granule. Methods: The HPLC system consisted of the FORTIS-C18 (4.6 mm×250 mm, 5 μm) column, the mobile phase consisted of acetonitrile and 0.2% H3PO4 gradient elution flow rate was 1.0 mL/min, the column temperature was 30 ℃, the UV detector was set at 330 nm. Results: The linear response ranges were 3.08~61.50 μg/mL and 2.18~43.60 μg/mL (r=0.999 9). The average recovery of orientin and isorientin were 99.1% and 98.6%, RSD were 0.94% and 0.90% respectively. Conclusion: The method is rapid, simple, reproducible and specific. It can be an important method to control the standard of Danzhuye granule. |
| Key words: orientin isorientin Danzhuye granule HPLC |
