| 摘要: |
| 目的:建立复方甘草口服溶液中甘草酸的含量测定方法。方法:采用高效液相色谱法(HPLC),色谱柱为Agilent Eclipse Plus-C18(250 mm×4.6 mm, 5 μm),流动相为甲醇-0.1%磷酸水溶液(70:30),检测波长250 nm,柱温25 ℃,流速1.0 mL/ min,进样量20 μL。结果:甘草酸浓度在29.18~182.35 μg/ mL 范围内时,样品浓度与其相应色谱峰峰面积积分值呈良好线性关系(r =0.999 2)。精密度、稳定性和重复性试验的相对标准偏差(RSD) 分别为0.59%、0.66% 和0.40%;样品平均加样回收率为97.47%,RSD为1.49%(n=9)。结论:该方法专属性、重复性和准确性均较好,可用于复方甘草口服溶液中甘草酸的含量测定。 |
| 关键词: 复方甘草口服溶液 甘草酸 高效液相色谱法 含量测定 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2016.10.017 |
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| 基金项目:南京医科大学科技发展基金项目,编号2012NJMU068。 |
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| Quantitative Determination of Compound Glycyrrhiza Oral Solution by HPLC Method |
| Zhan Wen, Xu Jin, Jing Xia, Sun Fang |
| (Nanjing Children's Hospital Affiliated to Nanjing Medical University, Jiangsu Nanjing 210008, China) |
| Abstract: |
| Objective: To establish a method of quantitative determination of glycyrrhizic acid in compound glycyrrhiza oral solution.Methods: HPLC analytic method was used. The Agilent Eclipse Plus-C18 column (250 mm×4.6mm, 5 μm) was used at room temperature (25 ℃) and eluted by methanol-0.1% phosphoric acid solution (70 :30) with a flow rate of 1.0 mL/ min. The detection wavelength was 250 nm and the injection volume was 20 μL. Results: A good linear relationship was obtained between the peak areas and the concentrations of glycyrrhizic acid ranging from 29.18~182.35 μg/ mL (r=0.9992). RSDs for precision, stability and reproducibility tests were 0.59%, 0.66% and 0.40%, respectively. The average recovery was 97.47% (RSD=1.49%, n=9). Conclusion: The HPLC method which was specific, accurate and reproducible could be used for quantitative determination of glycyrrhizic acid in compound glycyrrhiza oral solution. |
| Key words: compound glycyrrhiza oral solution glycyrrhizic acid HPLC quantitative determination |
