| 摘要: |
| 目的:探讨11β-羟基类固醇脱氢酶(11β-HSD)与促炎性细胞因子在川崎病(KD) 发生、发展中的变化及意义。方法:应用Real-time PCR检测KD患儿急性期及治疗后外周血单核细胞内11β-HSD mRNA的表达,应用免疫印迹方法检测11β-HSD 蛋白的表达;应用酶联免疫吸附试验检测血浆IL-17A和IL-6水平。结果:病例组患儿治疗前外周血单核细胞内11β-HSD1 mRNA表达水平及IL-17A和IL-6水平分别为7.13±0.79、(43.40±5.20) pg/ mL、(68.30±6.26) pg/ mL,较正常对照组1.00±0.07、(24.30±2.26) pg/ mL、(30.04±2.86) pg/ mL明显升高( P <0.01),而治疗后结果分别为3.43±0.52、(27.30±2.50) pg/ mL、(38.30±3.50)pg/ mL,均较治疗前明显下降(P<0.01);治疗前11β-HSD2 mRNA表达水平为0.32±0.05,明显低于正常对照组1.00±0.06(P<0.01),治疗后水平为0.82±0.04, 较治疗前明显升高(P<0.01)。免疫印迹检测11β-HSD蛋白表达水平的结果
分析与11β-HSD mRNA表达水平结果分析一致。结论:KD急性期11β-HSD、促炎性细胞因子在调节KD炎症反应中发挥着重要作用。 |
| 关键词: 川崎病 11β-羟基类固醇脱氢酶 促炎性细胞因子 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2016.08.001 |
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| 基金项目:西安市卫生局科技项目,编号J2011031。 |
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| Significance of 11β-Hydroxysteroid Dehydrogenase and Proinflammatory Cytokines in Kawasaki Disease |
| Wang Juanli1 , Zhou Nan1 , Wu Shouzhen1 , Yan Xiaohua2 , Wang Tao1 , Xing Haijian1 |
| (1. Xi'an Children's Hospital, Shaanxi Xi'an 710003, China; 2. Shaanxi Provincial People's Hospital, Shaanxi Xi'an 710068, China) |
| Abstract: |
| Objective: To investigate the changes and significance of 11β-hydroxysteroid dehydrogenase (11β-HSD) and proinflammatory cytokines in the occurrence and development of Kawasaki disease (KD). Methods: Real-time PCR was performed to detect the mRNA expressions of 11β-HSD in peripheral blood monocytes in acute phase and after treatment. Western blot was performed to detect the protein expressions of 11β-HSD. ELISA was performed to detect the levels of IL-6 and IL-17 in plasma. Results: The expression levels of 11β-HSD1 mRNA, IL-17A and IL-6 in acute phase were 7.13±0.79、(43.40±5.20) pg/ mL、(68.30±6.26) pg/ mL, significantly higher than the normal levels 1.00±0.07、(24.30±2.26) pg/ mL、(30.04±2.86) pg/ mL ( P<0.01), after treatment the levels were 3.43±0.52、(27.30±2.50) pg/ mL、(38.30±3.50)pg/ mL, which were significantly reduced than before (P<0.05). The expression level of 11β-HSD2 mRNA was 0.32±0.05 before treatment, lower than the normal group (P<0.01), while significantly increased after treatment than before (P<0.01). The results of Western blot were consistent with those of Real-time PCR. Conclusion: 11β-HSD and the level of proinflammatory cytokines in acute phase of KD are important factors in regulating inflammatory responses. |
| Key words: Kawasaki disease 11β-hydroxysteroid dehydrogenase proinflammatory cytokines |
