| 摘要: |
| [摘要]目的：建立同时测定金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ的HPLC波长切换法。方法：选用Agilent TC-C18（250 mm×4.6 mm，5 µm）色谱柱；流动相：乙腈-0.2%磷酸溶液（梯度洗脱）；流速0.9 mL/min；柱温30 ℃；进样量20 µL。结果：金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ分别在6.12~122.40、3.78~75.60、3.35~67.00、12.76~255.20、8.94~178.80 µg/mL浓度范围内线性关系良好，平均回收率（n=6）分别为98.40%（RSD=1.71%）、97.56%（RSD=1.24%）、96.94%（RSD=0.88%）、100.19%（RSD=0.99%）、98.97%（RSD=1.03%）。结论：该方法简便、准确、重复性好，可为金衣万应丸多指标质量评价提供参考。 |
| 关键词: 金衣万应丸 波长切换法 儿茶素 表儿茶素 胡黄连苷Ⅲ 胡黄连苷Ⅱ 胡黄连苷Ⅰ |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2018.06.017 |
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| 基金项目: |
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| Simultaneous Determination on Effective Components of Jinyi Wanying Pills by HPLC Wavelength Switching Method |
| Ma Danfeng1, Li Sujuan2, Liu Wen3 |
| (1. Hunan Children’s Hospital, Hunan Changsha 410007, China; 2. People’s Hospital of Dongxihu District Wuhan City, Hubei Wuhan 430040, China; 3. Wuhan Fourth Hospital, Hubei Wuhan 430033, China) |
| Abstract: |
| [Abstract] Objective: To establish the quantitative method of (+)-catechin, epicatechin, picroside Ⅲ, amphicoside Ⅱ and amphicosideⅠ in Jinyi Wanying pills by HPLC wavelength switching method. Methods: The determination was performed on chromatographic column of Agilent TC-C18 (250 mm×4.6 mm, 5 µm). The mobile phase consisted of acetonitrile (A)-0.2% of phosphoric acid solution (B) with gradient elution. The flow rate was 0.9 mL/min. The column temperature was set at 30℃. The injection volume was 20 µL. Results: The concentration ranges of (+)-catechin, epicatechin, picroside Ⅲ, amphicoside Ⅱ and amphicosideⅠ were respectively 6.12-122.40, 3.78-75.60, 3.35-67.00, 12.76-255.20, 8.94-178.80 µg/mL, with well linear relation. The average recovery of the above five components were 98.40% (RSD=1.71%), 97.56% (RSD=1.24%), 96.94% (RSD=0.88%), 100.19% (RSD=0.99%), 98.97% (RSD=1.03%), respectively. Conclusion: This determination method is convenient and accurate, with good repeatability, which can provide references for multi-indices quality evaluation of Jinyi Wanying pills. |
| Key words: Jinyi Wanying Wan wavelength switching method (+)-catechin epicatechin picroside-Ⅲ amphicoside Ⅱ amphicosideⅠ |
