| 摘要: |
| [摘要]目的：探讨Th9、IL-9和IFN-γ在肺炎支原体肺炎（MPP）患儿发病中的作用及其与肺功能变化的相关性。方法：选择100例MPP患儿，其中喘息组42例，非喘息组58例；另选体检健康的儿童25例为对照组。采用流式细胞术检测外周血Th9细胞比例，采用ELISA测定IL-9和IFN-γ水平；采用肺功能仪检测肺功能，检测指标为第1秒用力呼气容积占预计值的百分比（FEV1%-pred）、第1秒用力呼气容积占用力肺活量的百分比（FEV1/FVC%）及残气量占预计值的百分比（RV%-pred）。采用Pearson检验分析检查结果的相关性。结果：喘息组患儿的FEV1%-pred和FEV1/FVC%低于非喘息组患儿，RV%-pred、Th9、IL-9、IFN-γ水平均高于非喘息组患儿（P均<0.05）。MPP患儿的Th9细胞比例（r分别为-0.962 4、-0.961 1）、IL-9水平（r分别为-0.951 0、-0.958 0）、IFN-γ水平（r分别为-0.960 9、-0.962 9）与FEV1%-pred和FEV1/FVC%均呈负相关，与RV%-pred呈正相关（r分别为0.978 9、0.982 5、0.963 5）。结论：MPP患儿外周血Th9、IL-9和IFN-γ水平明显升高，且升高程度随着患儿出现喘息而加大，这种变化可为临床判断患儿肺功能降低提供参考。 |
| 关键词: 肺炎支原体 肺炎 Th9细胞 白介素-9 IFN-γ 肺功能 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2018.06.002 |
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| 基金项目: |
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| Changes and Significance of Th9, IL-9 and IFN-γ Levels in Peripheral Blood of Children with Mycoplasma Pneumoniae Pneumonia |
| Shen Jin, Shen Guoqiang, Wang Xiaoming, Sun Shaojun, Yang Qianyun |
| (The Ninth People’s Hospital of Wuxi City, Jiangsu Wuxi 214000, China) |
| Abstract: |
| [Abstract] Objective: To investigate the role of Th9, IL-9 and IFN-γ levels in children with Mycoplasma Pneumoniae pneumonia (MPP) and its correlation with pulmonary function. Methods: A total of 100 children with MPP were divided into asthma group (n=42) and non-asthma group (n=58); and another 25 healthy children were extracted as control group. The percentage of Th9 cells in peripheral blood were detected by flow cytometry and IL-9 and IFN-γ levels were measured by ELISA. Pulmonary function was determined by spirometer, with the testing indicators of FEV1%-pred, FEV1/FVC% and RV%-pred. Correlation of the results of pulmonary function indicators were analyzed by Pearson analysis. Results: The FEV1%-pred and FEV1/FVC% in asthma group were lower than those in non-asthma group, and the RV%-pred, Th9, IL-9 and IFN-γ levels were higher than those in non-asthma group(P<0.05). The ratio of Th9 cells (r were respectively -0.962,4 and -0.961,1), IL-9 levels (r were respectively -0.951,0 and -0.958,0), IFN-γ levels (r were respectively -0.960,9 and -0.962,9) showed significant negative correlation with FEV1%-pred and FEV1/FVC% and had positive correlation with RV%-pred (r were respectively 0.978,9, 0.982,5 and 0.963,5) in children with MPP. Conclusion: The Th9, IL-9 and IFN-γ levels in peripheral blood of children with MPP increased significantly, and the degree is aggravating with the pulmonary wheezing, which can provide reference for clinical judgment of pulmonary function decline in children. |
| Key words: Mycoplasma pneumoniae pneumonia Th9 cells IL-9 IFN-γ pulmonary function |
