| 摘要: |
| 目的：考察金振口服液的退热作用及其作用机制。方法：采用酵母致大鼠发热模型，测定大鼠注射酵母后6、7、8 h时的肛温，放射免疫法检测大鼠血清IL-6、TNF-α含量及下丘脑组织5-HT、NE含量。采用腹腔注射脂多糖（LPS）复制大鼠发热模型，测定模型大鼠肛温，放射免疫法检测模型大鼠血清TNF-α、IL-1β含量及下丘脑组织环腺苷酸（cAMP）、前列腺素E2（PGE2）含量。实验过程中，以金振口服液为干预药物，分高（4.4 g/kg）、中（2.2 g/kg，相当于临床等效剂量）、低（1.1 g/kg）三个剂量水平，以布洛芬0.05 g/kg为阳性对照。结果：金振口服液高剂量组大鼠在注射酵母后7、8 h时的体温均低于模型对照组，中剂量组大鼠在注射酵母后8 h时的体温低于模型对照组（P均<0.05）；模型对照组大鼠血清TNF-α、IL-6含量均高于正常对照组（P均<0.05）；金振口服液高、中、低剂量组大鼠血清TNF-α含量及高、中剂量组血清IL-6含量均低于模型对照组（P均<0.05）；模型对照组大鼠下丘脑中5-HT含量高于正常对照组，NE含量低于正常对照组（P均<0.05）；金振口服液高、中剂量组大鼠下丘脑中5-HT含量均低于模型对照组，高剂量组大鼠下丘脑中NE含量高于模型对照组（P均<0.05）。模型对照组大鼠在注射LPS后2、3、4、5、6 h时的体温均高于正常对照组（P均<0.05）；金振口服液高剂量组大鼠在注射LPS后2、3、4、5、6 h时的体温均低于模型对照组，中剂量组大鼠在注射LPS后3、4、6 h时的体温均低于模型对照组（P均<0.05）；模型对照组大鼠血清IL-1β、TNF-α含量及下丘脑中cAMP、PGE2含量均高于正常对照组（P均<0.05）；金振口服液高、中、低剂量组大鼠血清TNF-α、IL-1β含量均低于模型对照组（P均<0.05）；金振口服液高、中剂量组大鼠下丘脑中cAMP、PGE2含量均低于模型对照组（P均<0.05）。结论：金振口服液具有显著的解热作用，其作用机制可能是通过降低血清中炎症因子，从而抑制下丘脑中致热介质的产生而发挥作用。 |
| 关键词: 解热 脂多糖 单胺类神经递质 环腺苷酸 前列腺素E2 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2021.02.001 |
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| 基金项目: |
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| Animal Experimental Study on Antipyretic Mechanism of Jinzhen Oral Liquid |
| Hong Qian, Chen Changxiu, Liu Yanxia, Wang Yang, Li Yanli |
| (Huaihai Hospital Affiliated to Xuzhou Medical University, The 71st Group Army Hospital of CPLA Army, Jiangsu Xuzhou 221004, China) |
| Abstract: |
| Objective: To investigate the antipyretic effect of Jinzhen oral liquid, and explore the underlying mechanism. Methods: The model of yeast-induced fever in rat was used to determine the rectal temperature at 6, 7 and 8 h after yeast injection. The contents of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum and 5-hydroxytryptamine (5-HT) and noradrenaline (NE) in hypothalamus were detected by radioimmunoassay. The model of yeast-induced fever in rat was established by intraperitoneal injection of lipopolysaccharide (LPS), rectal temperature of the rats was determined, the contents of TNF-α and IL-1β in serum, cyclic adenosine monophosphate (cAMP) and prostaglandin E2 (PGE2) in hypothalamus were detected by radioimmunoassay. During the experiment, Jinzhen oral solution was used as the intervention drug, and the intervention drug was divided into three groups: high (4.4 g/kg), medium (2.2 g/kg, clinical equivalent dose) and low (1.1 g/kg), and ibuprofen 0.05 g/kg was set as the positive control. Results: The body temperature of rats in Jinzhen oral liquid high-dose group at 7 and 8 h after yeast injection was lower than that of the model control group, and the body temperature of rats in medium-dose group at 8 h after yeast injection was lower than that of the model control group (P<0.05). Serum contents of TNF-α and IL-6 in the model control group were higher than those in the normal control group (P<0.05). The serum content of TNF-α in Jinzhen oral liquid high-dose, medium-dose and low-dose group and the serum content of IL-6 in high-dose and medium-dose group were lower than those of the model control group (P<0.05). The content of 5-HT in hypothalamus of the model control group was higher than that of the normal control group, and the content of NE was lower than that of the normal control group (P<0.05). The content of 5-HT in hypothalamus of rats in Jinzhen oral liquid high-dose and medium-dose group was lower than that in the model control group, and the content of NE in hypothalamus of rats in the high-dose group was higher than that in the model control group (P<0.05). The body temperature of the model control group at 2, 3, 4, 5 and 6 h after LPS injection was higher than that of the normal control group (P<0.05). The body temperature of rats in Jinzhen oral liquid high-dose group at 2, 3, 4, 5 and 6 h after LPS injection was lower than that of the model control group, and the body temperature of rats in the medium-dose group at 3, 4 and 6 h after LPS injection was lower than that of the model control group (P<0.05). The contents of IL-1β and TNF-α in serum and the contents of cAMP and PGE2 in hypothalamus in the model control group were higher than those in the normal control group (P<0.05). The contents of serum TNF-α and IL-1β in Jinzhen oral liquid high-dose, medium-dose and low-dose group were lower than those in the model control group (P<0.05). The contents of cAMP and PGE2 in hypothalamus of rats in Jinzhen oral liquid high-dose and medium-dose group were lower than those in the model control group (P<0.05). Conclusion: Jinzhen oral liquid has significant antipyretic effect, and its mechanism of action may be to reduce the inflammatory factors in the serum, thereby inhibiting the production of pyrogenic media in the hypothalamus. |
| Key words: antipyretic lipopolysaccharide monoamine neurotransmitter cyclic adenosine monophosphate prostaglandin E2 |
