| 摘要: |
| [摘要]目的:建立测定人血清中奥卡西平活性代谢产物10，11-二氢-10-羟基卡马西平血药浓度的超高效液相色谱法。方法:采集静脉血样本，高氯酸沉淀蛋白，离心取上清液进样，色谱柱为Waters ACQUITY UPLCBEH C18（2.1 mm×50 mm，1.7 μm），流动相为0.05%甲酸水溶液﹕乙腈=75﹕25，检测波长254 nm，流速0.20 mL/min，柱温40 ℃。结果:10，11-二氢-10-羟基卡马西平在5~100 μg/mL浓度范围峰面积与内标峰面积比值对浓度比值的线性关系良好（r＝0.999 9），回收率、精密度、稳定性、准确度均在限度要求范围内。结论:该方法操作简单、准确、灵敏，可用于临床监测奥卡西平活性代谢产物10，11-二氢-10-羟基卡马西平血药浓度监测。 |
| 关键词: 癫痫 奥卡西平 10,11-二氢-10-羟基卡马西平 血药浓度 超高效液相色谱法 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2022.01.005 |
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| 基金项目: |
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| Determination for Blood Concentration of Oxcarbazepine Active Metabolite by Ultra-High Performance Liquid Chromatography |
| Zong Jing, Li Fashuang, Ru Yi, Yu Shaochun, Lei Yan, Li Huiying |
| (Kunming Children’s Hospital, Yunnan Kunming 650034, China) |
| Abstract: |
| [Abstract] Objective: To establish the ultra-high performance liquid chromatography method for the blood concentration determination of active metabolite of oxcarbazepine 10,11-dihydro-10-hydroxyl carbamazepine. Methods: Venous blood samples were collected, proteins were precipitated by perchloric acid, and the supernatant was centrifuged and fed into the sample. The determination was performed on Waters ACQUITY UPLCBEH C18 (2.1 mm×50 mm, 1.7 μm) with 0.05% formic acid aqueous solution∶acetonitrile =75∶25 as mobile phase. The detection wavelength was 254 nm, the flow rate was 0.20 mL/min, and the column temperature was 40 ℃. Results: The linearity of the peak area of 10, 11-dihydro-10-hydroxyl carbamazepine in the concentration range from 5 to 100 μg/mL and the peak area ratio of the internal standard to the concentration ratio were good (r=0.999,9), and the recoveries, precision, stability and accuracy were within the limit requirements. Conclusion: The method is simple, accurate and sensitive, which can be used for blood concentration monitoring of active metabolite of oxcarbazepine 10, 11-dihydro-10-hydroxyl carbamazepine. |
| Key words: epilepsy oxcarbazepine 10, 11-dihydro-10-hydroxyl carbamazepine blood concentration ultra-high performance liquid chromatography |
