| 摘要: |
| [摘要]目的：探讨microRNA-515-5p（miR-515-5p）对神经母细胞瘤（NB）SK-N-BE（2）和SK-N-AS细胞增殖、侵袭、迁移的影响及分子机制。方法：采用生物信息学技术结合多种软件预测方法，检测miR-515-5p在NB细胞中的表达。在NB细胞中瞬时转染miR-515-5p mimics，应用CCK8法和克隆形成实验、Transwell侵袭小室实验和体外划痕实验检测miR-515-5p对NB细胞转染后的增殖、侵袭和迁移的影响。采用Western免疫印迹（WesternBlot）检测miR-515-5p对c-MYC-p53信号轴的调控机制。结果：与对照组比较，转染miR-515-5p mimics的细胞集落平均形成率降低，细胞活性下降，且细胞侵袭和迁移能力降低（P<0.05）。WesternBlot检测结果显示miR-515-5p抑制c-MYC蛋白表达，但可促进P-p53活化（P<0.05），对p53的表达无影响。结论：miR-515-5p通过调控c-MYC-p53信号轴抑制NB细胞的增殖、侵袭和迁移能力，可能作为NB分子治疗的潜在靶点。 |
| 关键词: 神经母细胞瘤 microRNA-515-5p c-MYC-p53 侵袭 转移 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2021.10.001 |
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| 基金项目:苏州市高新区医疗卫生科技项目,编号2018Q012。 |
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| Effects of MicroRNA-515-5p on Proliferation, Invasion and Migration of Neuroblastoma Cells by Regulating c-MYC-p53 Signal Axis |
| Ji Shiliang, Hong Lei, Guo Dongkai, Jiang Yiguo, Xu Fei, Huang Lifeng |
| (The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Jiangsu Suzhou 215123, China) |
| Abstract: |
| [Abstract] Objective: To probe into the effects of microRNA-515-5p (miR-515-5p) on proliferation, invasion, migration and molecular mechanisms of SK-N-BE(2) and SK-N-AS of neuroblastoma (NB) cells. Methods: Bioinformatics techniques combined with multiple software prediction methods were used to detect the expression of miR-515-5p in NB. The miR-515-5p mimics were transiently transfected in NB cells, and effects of miR-515-5p on the proliferation, invasion and migration of NB cells after transfection were examined by CCK8 method and colony-forming unit assay, Transwell invasion cell assay and in vitro scratch assay. Western Blot was used to detect the regulatory mechanism of miR-515-5p on the c-MYC-p53 signal axis. Results: Compared with the control group, cells transfected with miR-515-5p mimics had the reduced mean colony formation rate, decreased cell activity, and reduced cell invasion and migration ability (P<0.05). Western Blot showed that miR-515-5p inhibited the expression of c-MYC protein, yet promoted the activation of P-p53 (P<0.05), and had no effect on the expression of p53. Conclusion: The miR-515-5p inhibits the proliferation, invasion and migration of NB cells by regulating the c-MYC-p53 signaling axis, which may be the potential target for molecular therapy of NB. |
| Key words: neuroblastoma microRNA-515-5p c-MYC-p53 invasion migration |
