| 摘要: |
| 目的:建立测定新生儿血清中咖啡因血药浓度的高效液相色谱法,并对使用咖啡因治疗原发性呼吸暂停新生儿的血药浓度进行测定。 方法:色谱柱为 ZORBAX Extend C18 柱,流动相为乙腈-水(19 ∶ 81,V/ V),柱温 25 ℃ ,流速 1 mL/ min,检测波长273 nm。 结果:咖啡因血药浓度在 1. 0~160. 0 μg / mL 范围内呈良好的线性关系(r = 0. 998 9),平均回收率 98. 40% ~ 102. 40%,日内与日间相对标准差(RSD)≤9. 63%,检测 10 例患儿给药 7 d 后咖啡因血药浓度为(20. 53±11. 12)μg / mL。 结论:建立的高效液相色谱法准确、快速,经验证适用于新生儿血清中咖啡因血药浓度测定。 |
| 关键词: 新生儿 咖啡因 高效液相色谱法 血药浓度 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2023.08.009 |
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| 基金项目: |
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| Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application |
| Li Sai1, Li Shoulin1, Su Lingying2 |
| (1.Huai’an Women and Children’s Health Care Hospital, Jiangsu Huai’an 223001, China; 2. The Affiliated Huai’an No. 1 People’s Hospital of Nanjing Medical University, Jiangsu Huai’an 223300, China) |
| Abstract: |
| Objective: To establish the high performance liquid chromatography (HPLC) method for the determination of caffeine blood concentration in neonates, and to determine the concentration of caffeine in neonates with primary apnea. Methods: The determination was performed on ZORBAX Extend C18
column with mobile phase acetonitrile-water (19 ∶ 81, V/ V) at the column temperature of 25 ℃ and flow rate of 1 mL/ min. The detection wavelength was 273 nm. Results: Caffeine blood concentration showed good linearity ( r =0. 998,9) in the range of 1. 0 to 160. 0 μg / mL, with mean recoveries of 98. 40% to 102. 40%. Inter-day and intra-day relative standard
deviation (RSD) was ≤9. 63%. The average caffeine blood concentration in 10 children was ( 20. 53± 11. 12) μg / mL after 7 d ofadministration. Conclusion: The established high performance liquid chromatography method is accurate, rapid and validated for the determination of caffeine blood concentration in neonates. |
| Key words: neonates caffeine high performance liquid chromatography blood concentration |
