| 摘要: |
| 目的:探讨宏基因组二代测序(mNGS)技术在新生儿中枢神经系统感染病原学诊断中的应用价值。 方法:选择 2019 年 1 月至 2023 年 6 月我院新生儿重症监护室(NICU)诊断为中枢神经系统感染的新生儿 82 例,分别进行 mNGS 检测与常规检测 后,比较分析检测结果,评价抗菌药物的使用对检出率的影响。 结果:82 例新生儿中,化脓性脑膜炎 73 例,病毒性脑炎 8 例,真 菌性脑膜炎 1 例。 脑脊液 mNGS 共检出 41 例(50. 00%)阳性,其中细菌 36 例,病毒 4 例,真菌 1 例;常规病原学共检出 12 例 (14. 63%)阳性,其中细菌 10 例,病毒 2 例。 脑脊液 mNGS 检出病原体种类与常规病原学基本一致,但检出率较高(P<0. 05)。 使用抗菌药物后,脑脊液 mNGS 致病菌检测的阳性率为 47. 37%,高于常规病原学检测的 10. 52%(P<0. 05)。 结论:mNGS 较常 规病原学检测可提高新生儿中枢神经系统感染病原体的检出率,且不易受使用抗菌药物的影响,具有较高的临床应用价值,能 有效指导临床诊疗与用药。 |
| 关键词: 新生儿 感染 中枢神经系统 宏基因组二代测序 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2026.03.010 |
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| 基金项目:徐州市卫生健康委员会医学科技创新项目,编号 XWKYHT20220087;徐州市儿童医院课题,编号 23040440。 |
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| Metagenomics Next-Generation Sequencing Technology in Assisting Pathogen Diagnosis and Drug Treatment of Neonatal Central Nervous System Infection |
| Peng Bo, Liang Hongying, Wang Shihui, Liu Gang, Wang Wei, Liu Jinfeng |
| (Xuzhou Children’ s Hospital Affiliated to Xuzhou Medical University, Jiangsu Xuzhou 221006, China) |
| Abstract: |
| Objective: To explore the application value of metagenomics next-generation sequencing ( mNGS) technology in the pathogenic diagnosis of neonatal central nervous system infection. Methods: Totally 82 newborns diagnosed with central nervous system infection in the Neonatal Intensive Care Unit (NICU) of our hospital from Jan. 2019 to Jun. 2023 were selected. After mNGS detection and routine detection, the results were compared and analyzed to evaluate the effects of antibiotics on the detection rate. Results: Among the 82 newborns, 73 cases had purulent meningitis, 8 cases had viral meningitis, and 1 case had fungal meningitis. Cerebrospinal fluid mNGS detected 41 cases (50. 00%) as positive, including 36 cases of bacteria, 4 cases of virus, and 1 case of fungi; conventional pathogen detection detected 12 cases (14. 63%) as positive, including 10 cases of bacteria and 2 cases of virus. The types of pathogens detected by cerebrospinal fluid mNGS were basically the same as those detected by conventional pathogen detection, but the detection rate was higher (P<0. 05). After the use of antibiotics, the positive rate of pathogenic bacteria detected by cerebrospinal fluid mNGS was 47. 37%, higher than 10. 52% obtained from conventional pathogen detection ( P < 0. 05). Conclusion: Compared with the conventional pathogen detection, mNGS can significantly improve the detection rate of pathogens in neonatal central nervous system infection. Moreover, it is hardly affected by the use of antibiotics and has high clinical application value, which can effectively guide clinical diagnosis and treatment. |
| Key words: newborns infection central nerves system metagenomics next-generation sequencing |
