| 摘要: |
| 目的:探讨特应性皮炎(AD)患儿外周血滤泡辅助T 细胞13(Tfh13)水平及其临床意义,并结合血清蛋白质组学分析,为
精准用药提供依据。方法:纳入16 例AD 患儿与18 例健康对照儿童,采用流式细胞术检测外周血Tfh13 及Tfh2 细胞比例;
Olink 蛋白质组学技术检测血清炎症蛋白。结果:AD 患儿外周血Tfh13、Tfh2 比例显著高于对照组,合并哮喘/ 过敏性鼻炎或血
清总IgE 升高的患儿Tfh13 比例更高,而Tfh2 在上述分层中差异无统计学意义。Tfh13 细胞比例与总IgE 呈正相关,与AD 积分
指数(SCORAD)评分无相关性。蛋白质组学显示,伴过敏性共病AD 患儿血清白细胞介素( IL)-4、ADA、IL-8、CX3CL1 水平升
高,而IL-13、TARC 等水平比较差异无统计学意义。结论:Tfh13 细胞可能在伴过敏性共病的AD 表型及IgE 介导的免疫应答中
发挥作用,AD 伴过敏性共病患儿存在炎症网络活化蛋白谱(IL-4/ ADA/ IL-8/ CX3CL1),为靶向IL-4/ IL-13 通路及JAK 抑制剂的
精准用药提供了潜在生物标志物。 |
| 关键词: 特应性皮炎 滤泡辅助T 细胞13 过敏性共病 精准用药 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2026.05.008 |
|
| 基金项目:基金项目:重庆市自然科学基金面上项目,编号CSTB2022NSCQ-MSX0906。 |
|
| Significance of Peripheral Blood Follicular Helper T Cell 13 Subset Levels in Children with AtopicDermatitis and Clinical Diagnosis and Treatment |
| Zhou Xiaoying, Wang Hua, Xiao Yizhu, Jiang Jinqiu |
| (Department of Dermatology, Children’s Hospital of Chongqing Medical
University, National Clinical Research Center for Children and Adolescents’ Health and Diseases, Ministry of Education Key Laboratory of
Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Chongqing 400014,
China) |
| Abstract: |
| Objective: To investigate the peripheral blood follicular helper T cell 13 (Tfh13) subset in children with atopic dermatitis
(AD) and its clinical significance, and to provide basis for precision medication through serum proteomic analysis. Methods: A total of
16 children with AD and 18 healthy controls were enrolled. Flow cytometry was used to detect the proportions of peripheral blood Tfh13
and Tfh2 cells, and Olink proteomic technology was employed to measure serum inflammatory proteins. Results: The proportions of
Tfh13 and Tfh2 cells in peripheral blood were significantly higher in children with AD than those in healthy controls. Among AD
patients, those with comorbid asthma/ allergic rhinitis or elevated serum total IgE levels showed a significantly higher proportion of Tfh13
cells, whereas no statistically significant differences in Tfh2 proportion were observed across these subgroups. Tfh13 proportion was
positively correlated with total IgE levels, but not correlated with SCORAD scores. Proteomic analysis revealed elevated levels of IL-4,
ADA, IL-8, and CX3CL1 in AD children with allergic comorbidities, whereas no statistically significant differences were found in IL-13,
TARC, or other classical Th2 markers. Conclusion: Tfh13 cells may play a role in the allergic phenotype of AD and IgE-mediated
immune responses, particularly in children with allergic comorbidities. Children with AD accompanied by allergic comorbidities have an
activated inflammatory network protein profile (IL-4/ ADA/ IL-8/ CX3CL1), providing potential biomarkers for targeted IL-4/ IL-13
pathway and JAK inhibitor-based precise medication. |
| Key words: atopic dermatitis follicular helper T cell 13 allergic comorbidities precision medication |
