| 摘要: |
| 目的:建立高效液相色谱(HPLC)测定川芎配方颗粒中阿魏酸含量的方法。方法:Agilent HC-C18色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇-1%冰醋酸溶液(30∶70),流速1.0 mL/min,检测波长321 nm,柱温25 ℃,进样量10 μL。结果:阿魏酸在2.712~29.832 μg/mL范围内与峰面积线性关系良好(r=0.999 8),平均回收率为101.02% (n=6),川芎配方颗粒中阿魏酸含量为1.45 mg/g。结论:该方法可用于医院川芎配方颗粒的质量控制。 |
| 关键词: 高效液相色谱 川芎配方颗粒 阿魏酸 |
| DOI：10.13407/j.cnki.jpp.1672-108X.2017.06.017 |
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| 基金项目: |
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| Determination of Content of Ferulic Acid in Ligusticum Chuanxiong Hort Formula Granule by HPLC |
| Li Xingui |
| (Anhui Provincial Children爷s Hospital, Anhui Hefei 230051, China) |
| Abstract: |
| Objective: To establish high performance liquid chromatography (HPLC) method for determinate the content of ferulic acid in Ligusticum chuanxiong hort formula granule. Methods: The column of Agilent HC-C18(250 mm×4.6 mm, 5 μm), methanol-1% HAC (30∶70) as mobile phase at the flow rate of 1.0 mL/min, the detection wavelenth was 321 nm, the temperature of column was 25 ℃, the injection volume was 10 μL. Results: The range of linearity was 2.712~29.832 μg/mL (r=0.999,8), the average recovery was 102.6% (n=6), the content of sample was 1.45 mg/g in average. Conclusion: HPLC method can be used to assay the ferulic acid in Ligusticum chuanxiong hort formula granule in hospital. |
| Key words: HPLC Ligusticum chuanxiong hort formula granule ferulic acid |
