| 摘要: |
| 目的：建立高效液相色谱-荧光检测法测定性早熟女童血浆中双酚A和4-壬基酚含量的方法。方法：采用Shim-pack VP-ODS C18色谱柱（150 mm×4.6 mm，5 μm），流动相为乙腈∶水=70∶30，流速1 mL/min，柱温30 ℃，检测激发波长275 nm，发射波长315 nm，外标法定量检测血浆中双酚A和4-壬基酚含量。结果：双酚A和4-壬基酚分别在1.45~5 937.50 ng/mL （r2=0.999 9，n=7），1.53~6 250.00 ng/mL（r2=0.998 8，n=7）范围内线性关系良好；日内、日间RSD均<7.83%，方法回收率均>94.6%。结论：本法简便、快速、灵敏、准确，适用于同时测定性早熟女童血浆中双酚A和4-壬基酚的含量和临床血样常规检测。 |
| 关键词: 高效液相色谱-荧光检测法 性早熟 双酚A 4-壬基酚 |
| DOI：doi:10.13407/j.cnki.jpp.1672-108X.2020.06.012 |
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| 基金项目: |
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| Determination of Bisphenol A and 4-Nonylphenol in Plasma of Girls with Precocious Puberty by High Performance Liquid Chromatography-Fluorescence Detection |
| Xiong Youjian, Hu Rong, Tu Qiong, Lu Qionghong |
| (Jiangxi Provincial Children’s Hospital, Jiangxi Nanchang 330006, China) |
| Abstract: |
| Objective: To establish the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of bisphenol A (BPA) and 4-nonylphenol (4-NP) in plasma of of girls with precocious puberty. Methods: Shim-pack VP-ODS C18 chromatographic column (150 mm×4.6 mm, 5 μm) was adopted, the mobile phase was acetonitrile∶water=70∶30, with flow rate of 1 mL/min, column temperature of 30 ℃, detection excitation wavelength of 275 nm, and emission wavelength of 315 nm. Content of BPA and 4-NP in plasma was detected by external standard method. Results: The linear relationship between BPA and 4-NP was good in the range from 1.45 to 5,937.50 ng/mL (r2=0.999,9, n=7) and from 1.53 to 6,250.00 ng/mL (r2=0.998,8, n=7). The RSD of intra-day and inter-day precision was <7.83%, and the recovery rate was >94.6%. Conclusion: This method is simple, rapid, sensitive and accurate, and is suitable for simultaneous determination of BPA and 4-NP in plasma and routine detection of clinical blood samples in girls with precocious puberty. |
| Key words: high performance liquid chromatography-fluorescence detection precocious puberty bisphenol A 4-nonylphenol |
